CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-020898
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Ethylmalonyl-CoA decarboxylase 
Protein Synonyms/Alias
 Enoyl-CoA hydratase domain-containing protein 1; Methylmalonyl-CoA decarboxylase; MMCD 
Gene Name
 Echdc1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
75GSIDLLKKQNGIGILubiquitination[1]
232KVLSGTLKLDSKEALacetylation[2, 3, 4, 5]
232KVLSGTLKLDSKEALsuccinylation[4]
232KVLSGTLKLDSKEALubiquitination[1]
316ANLEAIAKKGKHTK*acetylation[3, 4]
316ANLEAIAKKGKHTK*succinylation[4]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123]
 [3] Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways.
 Rardin MJ, Newman JC, Held JM, Cusack MP, Sorensen DJ, Li B, Schilling B, Mooney SD, Kahn CR, Verdin E, Gibson BW.
 Proc Natl Acad Sci U S A. 2013 Apr 16;110(16):6601-6. [PMID: 23576753]
 [4] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [5] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
 Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
 J Biol Chem. 2013 Jul 17;. [PMID: 23864654
Functional Description
 Decarboxylases ethylmalonyl-CoA decarboxylase, a potentially toxic metabolite, to form butyryl-CoA, suggesting it might be involved in metabolite proofreading. Also has methylmalonyl-CoA decarboxylase activityx at lower level. 
Sequence Annotation
  
Keyword
 Alternative splicing; Complete proteome; Cytoplasm; Lyase; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 322 AA 
Protein Sequence
MRRCEVNSKP ISEYFGIPCE NREMAKCLLT SSLSVRTKLL QTGVSLYNTS HGFHEEEVKK 60
ILEQFPGGSI DLLKKQNGIG ILTLNNPNKM NAFSGVMMLQ LLERVIELEN WTEGKGLIIH 120
GAKNTFCSGS DLNAVKALST PESGVALSMF MQNTLTRFMR LPLISVALVQ GWAMGGGAEL 180
TTACDFRLMT EESVIRFVHK EMGIVPSWGG TSRLVEIIGS RQALKVLSGT LKLDSKEALN 240
IGLTDEVLQP SDETTALEQA QEWLEKFVSG PPQVIRGLKK SVCSARELYI EEALQNERDV 300
LETLWGGPAN LEAIAKKGKH TK 322 
Gene Ontology
 GO:0005829; C:cytosol; IDA:UniProtKB.
 GO:0016831; F:carboxy-lyase activity; IDA:UniProtKB.
 GO:0004492; F:methylmalonyl-CoA decarboxylase activity; IEA:EC. 
Interpro
 IPR001753; Crotonase_core_superfam.
 IPR018376; Enoyl-CoA_hyd/isom_CS. 
Pfam
 PF00378; ECH 
SMART
  
PROSITE
 PS00166; ENOYL_COA_HYDRATASE 
PRINTS