CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-000928
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Endothelin-converting enzyme 2 
Protein Synonyms/Alias
 ECE-2; Methyltransferase-like region; Endothelin-converting enzyme 2 region 
Gene Name
 ECE2 
Gene Synonyms/Alias
 KIAA0604; UNQ403/PRO740 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
116WETMDVRKLDFPSASubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961
Functional Description
 Converts big endothelin-1 to endothelin-1. Also involved in the processing of various neuroendocrine peptides, including neurotensin, angiotensin I, substance P, proenkephalin-derived peptides, and prodynorphin-derived peptides. May limit beta- amyloid peptide accumulation in brain. May also have methyltransferase activity. 
Sequence Annotation
 REGION 1 160 Methyltransferase-like region.
 REGION 88 89 S-adenosyl-L-homocysteine binding.
 REGION 113 114 S-adenosyl-L-homocysteine binding.
 REGION 200 883 Endothelin-converting enzyme 2 region.
 ACT_SITE 721 721 By similarity.
 ACT_SITE 784 784 Proton donor (By similarity).
 METAL 720 720 Zinc; catalytic (By similarity).
 METAL 724 724 Zinc; catalytic (By similarity).
 METAL 780 780 Zinc; catalytic (By similarity).
 BINDING 30 30 S-adenosyl-L-homocysteine.
 BINDING 41 41 S-adenosyl-L-homocysteine; via amide
 BINDING 66 66 S-adenosyl-L-homocysteine; via carbonyl
 BINDING 130 130 S-adenosyl-L-homocysteine.
 MOD_RES 39 39 Phosphotyrosine.
 CARBOHYD 324 324 N-linked (GlcNAc...) (Potential).
 CARBOHYD 384 384 N-linked (GlcNAc...) (Potential).
 CARBOHYD 429 429 N-linked (GlcNAc...) (Potential).  
Keyword
 3D-structure; Alternative splicing; Complete proteome; Glycoprotein; Golgi apparatus; Hydrolase; Membrane; Metal-binding; Metalloprotease; Methyltransferase; Multifunctional enzyme; Phosphoprotein; Polymorphism; Protease; Reference proteome; Signal-anchor; Transferase; Transmembrane; Transmembrane helix; Zinc. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 883 AA 
Protein Sequence
MASPGAGRAP PELPERNCGY REVEYWDQRY QGAADSAPYD WFGDFSSFRA LLEPELRPED 60
RILVLGCGNS ALSYELFLGG FPNVTSVDYS SVVVAAMQAR HAHVPQLRWE TMDVRKLDFP 120
SASFDVVLEK GTLDALLAGE RDPWTVSSEG VHTVDQVLSE VGFQKGTRQL LGSRTQLELV 180
LAGASLLLAA LLLGCLVALG VQYHRDPSHS TCLTEACIRV AGKILESLDR GVSPCEDFYQ 240
FSCGGWIRRN PLPDGRSRWN TFNSLWDQNQ AILKHLLENT TFNSSSEAEQ KTQRFYLSCL 300
QVERIEELGA QPLRDLIEKI GGWNITGPWD QDNFMEVLKA VAGTYRATPF FTVYISADSK 360
SSNSNVIQVD QSGLFLPSRD YYLNRTANEK VLTAYLDYME ELGMLLGGRP TSTREQMQQV 420
LELEIQLANI TVPQDQRRDE EKIYHKMSIS ELQALAPSMD WLEFLSFLLS PLELSDSEPV 480
VVYGMDYLQQ VSELINRTEP SILNNYLIWN LVQKTTSSLD RRFESAQEKL LETLYGTKKS 540
CVPRWQTCIS NTDDALGFAL GSLFVKATFD RQSKEIAEGM ISEIRTAFEE ALGQLVWMDE 600
KTRQAAKEKA DAIYDMIGFP DFILEPKELD DVYDGYEISE DSFFQNMLNL YNFSAKVMAD 660
QLRKPPSRDQ WSMTPQTVNA YYLPTKNEIV FPAGILQAPF YARNHPKALN FGGIGVVMGH 720
ELTHAFDDQG REYDKEGNLR PWWQNESLAA FRNHTACMEE QYNQYQVNGE RLNGRQTLGE 780
NIADNGGLKA AYNAYKAWLR KHGEEQQLPA VGLTNHQLFF VGFAQVWCSV RTPESSHEGL 840
VTDPHSPARF RVLGTLSNSR DFLRHFGCPV GSPMNPGQLC EVW 883 
Gene Ontology
 GO:0030659; C:cytoplasmic vesicle membrane; IDA:UniProtKB.
 GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0004222; F:metalloendopeptidase activity; IDA:UniProtKB.
 GO:0008168; F:methyltransferase activity; IEA:UniProtKB-KW.
 GO:0007420; P:brain development; ISS:UniProtKB.
 GO:0010002; P:cardioblast differentiation; ISS:UniProtKB.
 GO:0007267; P:cell-cell signaling; TAS:UniProtKB.
 GO:0016486; P:peptide hormone processing; IDA:UniProtKB.
 GO:0006508; P:proteolysis; IEA:UniProtKB-KW. 
Interpro
 IPR024079; MetalloPept_cat_dom.
 IPR013216; Methyltransf_11.
 IPR000718; Peptidase_M13.
 IPR018497; Peptidase_M13_C.
 IPR008753; Peptidase_M13_N. 
Pfam
 PF08241; Methyltransf_11
 PF01431; Peptidase_M13
 PF05649; Peptidase_M13_N 
SMART
  
PROSITE
 PS00142; ZINC_PROTEASE 
PRINTS
 PR00786; NEPRILYSIN.