CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-037086
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 Cell division control protein 42 homolog 
Protein Synonyms/Alias
  
Gene Name
 CDC42 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
149EITHHCPKTPFLLVGubiquitination[1, 2, 3]
170DDPSTIEKLAKNKQKubiquitination[4, 5]
175IEKLAKNKQKPITPEubiquitination[2, 3, 4, 6]
177KLAKNKQKPITPETAubiquitination[1, 3, 5]
186ITPETAEKLARDLKAacetylation[5]
186ITPETAEKLARDLKAubiquitination[1, 3, 4, 5]
195ARDLKAVKYVECSALubiquitination[1, 3, 5, 6, 7]
205ECSALTQKGLKNVFDubiquitination[1, 3, 5]
208ALTQKGLKNVFDEAIubiquitination[4]
225ALEPPEPKKSRRCVLubiquitination[1, 4, 7, 8, 9]
226LEPPEPKKSRRCVLLubiquitination[1, 4, 7, 9]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [5] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [6] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [7] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
 [8] Mass spectrometric analysis of lysine ubiquitylation reveals promiscuity at site level.
 Danielsen JM, Sylvestersen KB, Bekker-Jensen S, Szklarczyk D, Poulsen JW, Horn H, Jensen LJ, Mailand N, Nielsen ML.
 Mol Cell Proteomics. 2011 Mar;10(3):M110.003590. [PMID: 21139048]
 [9] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; GTP-binding; Membrane; Nucleotide-binding; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 233 AA 
Protein Sequence
MSPDILHNKQ ISIGICTDYL DFSWVNYIHF KQLKNQWKVR RGDTGFARSA GRQNSSRQDS 60
NEWWSQFGEV CPTSWNEVFD NYAVTVMIGG EPYTLGLFDT AGQEDYDRLR PLSYPQTDVF 120
LVCFSVVSPS SFENVKEKWV PEITHHCPKT PFLLVGTQID LRDDPSTIEK LAKNKQKPIT 180
PETAEKLARD LKAVKYVECS ALTQKGLKNV FDEAILAALE PPEPKKSRRC VLL 233 
Gene Ontology
 GO:0005622; C:intracellular; IEA:InterPro.
 GO:0016020; C:membrane; IEA:UniProtKB-KW.
 GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
 GO:0007264; P:small GTPase mediated signal transduction; IEA:InterPro. 
Interpro
 IPR027417; P-loop_NTPase.
 IPR005225; Small_GTP-bd_dom.
 IPR001806; Small_GTPase.
 IPR003578; Small_GTPase_Rho. 
Pfam
 PF00071; Ras 
SMART
 SM00174; RHO 
PROSITE
 PS51420; RHO 
PRINTS
 PR00449; RASTRNSFRMNG.