CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-026395
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 Rho guanine nucleotide exchange factor 2 
Protein Synonyms/Alias
  
Gene Name
 ARHGEF2 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
64CNKSITAKEALICPTubiquitination[1]
89DTLANCTKVKQKQQKubiquitination[1]
96KVKQKQQKAALLKNNubiquitination[1]
101QQKAALLKNNTALQSubiquitination[1, 2, 3]
149RSSLSLAKSVSTTNIubiquitination[1, 3]
256LHHVRTLKIMTRLFRubiquitination[1]
337PSAEQMCKTYSEFCSubiquitination[2, 4]
354SKALKLYKELYARDKubiquitination[1]
394LVTQRITKYPLLISRubiquitination[1]
439EGIYQLEKGARLQEIubiquitination[1, 3]
461AQTPVPGKGPFGREEubiquitination[1]
473REELLRRKLIHDGCLubiquitination[1]
483HDGCLLWKTATGRFKacetylation[3]
595EAYLRRIKMELQQKDubiquitination[1, 2, 4]
601IKMELQQKDRALVELubiquitination[1]
667IREVEGLKDLLVGPGubiquitination[1, 4]
800AAPVAPEKQATELALubiquitination[1, 3]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Metal-binding; Reference proteome; Zinc. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 987 AA 
Protein Sequence
MCCCCGCCPL LAHLEQGSLR ERTREKEKMK EAKDARYTNG HLFTTISVSG MTMCYACNKS 60
ITAKEALICP TCNVTIHNRC KDTLANCTKV KQKQQKAALL KNNTALQSVS LRSKTTIRER 120
PSSAIYPSDS FRQSLLGSRR GRSSLSLAKS VSTTNIAGHF NDESPLGLRR ILSQSTDSLN 180
MRNRTLSVES LIDEAEVIYS ELMSDFEMDE KDFAADSWSL AVDSSFLQQH KKEVMKQQDV 240
IYELIQTELH HVRTLKIMTR LFRTGMLEEL HLEPGVVQGL FPCVDELSDI HTRFLSQLLE 300
RRRQALCPGS TRNFVIHRLG DLLISQFSGP SAEQMCKTYS EFCSRHSKAL KLYKELYARD 360
KRFQQFIRKV TRPAVLKRHG VQECILLVTQ RITKYPLLIS RILQHSHGIE EERQDLTTAL 420
GLVKELLSNV DEGIYQLEKG ARLQEIYNRM DPRAQTPVPG KGPFGREELL RRKLIHDGCL 480
LWKTATGRFK DVLVLLMTDV LVFLQEKDQK YIFPTLDKPS VVSLQNLIVR DIANQEKGMF 540
LISAAPPEMY EVHTASRDDR STWIRVIQQS VRTCPSREDF PLIETEDEAY LRRIKMELQQ 600
KDRALVELLR EKVGLFAEMT HFQAEEDGGS GMALPTLPRG LFRSESLESP RGERLLQDAI 660
REVEGLKDLL VGPGVELLLT PREPALPLEP DSGGNTSPGV TANGEARTFN GSIELCRADS 720
DSSQRDRNGN QLRSPQEEAL QRLVNLYGLL HGLQAAVAQQ DTLMEARFPE GPERREKLCR 780
ANSRDGEAGR AGAAPVAPEK QATELALLQR QHALLQEELR RCRRLGEERA TEAGSLEARL 840
RESEQARALL EREAEEARRQ LAALGQTEPL PAEAPWARRP VDPRRRSLPA GDALYLSFNP 900
PQPSRGTDRL DLPVTTRSVH RNFEDRERQE LGSPEERLQD SSDPDTGSEE EGSSRLSPPH 960
SPRDFTRMQD IPEETESRDG EAVASES 987 
Gene Ontology
 GO:0005622; C:intracellular; IEA:InterPro.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0005543; F:phospholipid binding; IEA:InterPro.
 GO:0005089; F:Rho guanyl-nucleotide exchange factor activity; IEA:InterPro.
 GO:0035556; P:intracellular signal transduction; IEA:InterPro.
 GO:0035023; P:regulation of Rho protein signal transduction; IEA:InterPro. 
Interpro
 IPR000219; DH-domain.
 IPR011993; PH_like_dom.
 IPR001849; Pleckstrin_homology.
 IPR002219; Prot_Kinase_C-like_PE/DAG-bd.
 IPR015721; RhoGEF-like. 
Pfam
 PF00169; PH
 PF00621; RhoGEF 
SMART
 SM00109; C1
 SM00233; PH
 SM00325; RhoGEF 
PROSITE
 PS50010; DH_2
 PS50003; PH_DOMAIN
 PS00479; ZF_DAG_PE_1
 PS50081; ZF_DAG_PE_2 
PRINTS