CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-003685
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Cytosolic phospholipase A2 beta 
Protein Synonyms/Alias
 cPLA2-beta; Phospholipase A2 group IVB 
Gene Name
 PLA2G4B 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
34LAVPYLDKPPTPLHFubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 
Functional Description
 Calcium-dependent phospholipase A2 that selectively hydrolyzes glycerophospholipids in the sn-2 position with a preference for arachidonoyl phospholipids. Has a much weaker activity than PLA2G4A. Isoform 3 has calcium-dependent activity against palmitoyl-arachidonyl-phosphatidylethanolamine and low level lysophospholipase activity but no activity against phosphatidylcholine. Isoform 5 does have activity against phosphatidylcholine. 
Sequence Annotation
 DOMAIN 1 97 C2.
 DOMAIN 246 781 PLA2c.
 ACT_SITE 335 335 Nucleophile (By similarity).
 ACT_SITE 615 615 Proton acceptor (By similarity).  
Keyword
 Alternative splicing; Calcium; Complete proteome; Cytoplasm; Endosome; Hydrolase; Lipid degradation; Lipid metabolism; Membrane; Metal-binding; Mitochondrion; Polymorphism; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 781 AA 
Protein Sequence
MAEAALEAVR SELREFPAAA RELCVPLAVP YLDKPPTPLH FYRDWVCPNR PCIIRNALQH 60
WPALQKWSLP YFRATVGSTE VSVAVTPDGY ADAVRGDRFM MPAERRLPLS FVLDVLEGRA 120
QHPGVLYVQK QCSNLPSELP QLLPDLESHV PWASEALGKM PDAVNFWLGE AAAVTSLHKD 180
HYENLYCVVS GEKHFLFHPP SDRPFIPYEL YTPATYQLTE EGTFKVVDEE AMEKAEVSRT 240
CLLTVRVLQA HRLPSKDLVT PSDCYVTLWL PTACSHRLQT RTVKNSSSPV WNQSFHFRIH 300
RQLKNVMELK VFDQDLVTGD DPVLSVLFDA GTLRAGEFRR ESFSLSPQGE GRLEVEFRLQ 360
SLADRGEWLV SNGVLVAREL SCLHVQLEET GDQKSSEHRV QLVVPGSCEG PQEASVGTGT 420
FRFHCPACWE QELSIRLQDA PEEQLKAPLS ALPSGQVVRL VFPTSQEPLM RVELKKEAGL 480
RELAVRLGFG PCAEEQAFLS RRKQVVAAAL RQALQLDGDL QEDEIPVVAI MATGGGIRAM 540
TSLYGQLAGL KELGLLDCVS YITGASGSTW ALANLYEDPE WSQKDLAGPT ELLKTQVTKN 600
KLGVLAPSQL QRYRQELAER ARLGYPSCFT NLWALINEAL LHDEPHDHKL SDQREALSHG 660
QNPLPIYCAL NTKGQSLTTF EFGEWCEFSP YEVGFPKYGA FIPSELFGSE FFMGQLMKRL 720
PESRICFLEG IWSNLYAANL QDSLYWASEP SQFWDRWVRN QANLDKEQVP LLKIEEPPST 780
AGRIAEFFTD LLTWRPLAQA THNFLRGLHF HKDYFQHPHF STWKATTLDG LPNQLTPSEP 840
HLCLLDVGYL INTSCLPLLQ PTRDVDLILS LDYNLHGAFQ QLQLLGRFCQ EQGIPFPPIS 900
PSPEEQLQPR ECHTFSDPTC PGAPAVLHFP LVSDSFREYS APGVRRTPEE AAAGEVNLSS 960
SDSPYHYTKV TYSQEDVDKL LHLTHYNVCN NQEQLLEALR QAVQRRRQRR PH 1012 
Gene Ontology
 GO:0005829; C:cytosol; IDA:UniProtKB.
 GO:0031901; C:early endosome membrane; IEA:UniProtKB-SubCell.
 GO:0005576; C:extracellular region; TAS:UniProtKB.
 GO:0005743; C:mitochondrial inner membrane; TAS:Reactome.
 GO:0005509; F:calcium ion binding; NAS:UniProtKB.
 GO:0047498; F:calcium-dependent phospholipase A2 activity; IDA:UniProtKB.
 GO:0005544; F:calcium-dependent phospholipid binding; NAS:UniProtKB.
 GO:0004622; F:lysophospholipase activity; NAS:UniProtKB.
 GO:0006987; P:activation of signaling protein activity involved in unfolded protein response; TAS:Reactome.
 GO:0019369; P:arachidonic acid metabolic process; NAS:UniProtKB.
 GO:0019722; P:calcium-mediated signaling; NAS:UniProtKB.
 GO:0044267; P:cellular protein metabolic process; TAS:Reactome.
 GO:0046475; P:glycerophospholipid catabolic process; IDA:UniProtKB.
 GO:0006954; P:inflammatory response; NAS:UniProtKB.
 GO:0007567; P:parturition; NAS:UniProtKB.
 GO:0006654; P:phosphatidic acid biosynthetic process; TAS:Reactome.
 GO:0036151; P:phosphatidylcholine acyl-chain remodeling; TAS:Reactome.
 GO:0036152; P:phosphatidylethanolamine acyl-chain remodeling; TAS:Reactome.
 GO:0036148; P:phosphatidylglycerol acyl-chain remodeling; TAS:Reactome.
 GO:0036150; P:phosphatidylserine acyl-chain remodeling; TAS:Reactome. 
Interpro
 IPR016035; Acyl_Trfase/lysoPLipase.
 IPR000008; C2_Ca-dep.
 IPR008973; C2_Ca/lipid-bd_dom_CaLB.
 IPR018029; C2_membr_targeting.
 IPR002642; LysoPLipase_cat_dom. 
Pfam
 PF00168; C2
 PF01735; PLA2_B 
SMART
 SM00239; C2
 SM00022; PLAc 
PROSITE
 PS50004; C2
 PS51210; PLA2C 
PRINTS