CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-002176
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Guanine nucleotide-binding protein G(s) subunit alpha isoforms short 
Protein Synonyms/Alias
 Adenylate cyclase-stimulating G alpha protein 
Gene Name
 GNAS 
Gene Synonyms/Alias
 GNAS1 
Created Date
 July 27, 2013 
Organism
 Bos taurus (Bovine) 
NCBI Taxa ID
 9913 
Lysine Modification
Position
Peptide
Type
References
8MGCLGNSKTEDQRNEubiquitination[1]
53LGAGESGKSTIVKQMubiquitination[1]
58SGKSTIVKQMRILHVubiquitination[1]
307KVLAGKSKIEDYFPEubiquitination[1]
338DPRVTRAKYFIRDEFubiquitination[1]
Reference
 [1] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724] 
Functional Description
 Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The G(s) protein is involved in hormonal regulation of adenylate cyclase: it activates the cyclase in response to beta-adrenergic stimuli. 
Sequence Annotation
 NP_BIND 47 54 GTP (By similarity).
 NP_BIND 198 204 GTP (By similarity).
 NP_BIND 223 227 GTP (By similarity).
 NP_BIND 292 295 GTP (By similarity).
 METAL 54 54 Magnesium (By similarity).
 METAL 204 204 Magnesium (By similarity).
 BINDING 366 366 GTP; via amide nitrogen (By similarity).
 MOD_RES 51 51 Phosphoserine (By similarity).
 MOD_RES 352 352 Phosphoserine (By similarity).
 LIPID 2 2 N-palmitoyl glycine.
 LIPID 3 3 S-palmitoyl cysteine.
 CROSSLNK 300 300 Glycyl lysine isopeptide (Lys-Gly)  
Keyword
 3D-structure; Alternative splicing; Cell membrane; Complete proteome; GTP-binding; Isopeptide bond; Lipoprotein; Magnesium; Membrane; Metal-binding; Nucleotide-binding; Palmitate; Phosphoprotein; Reference proteome; Transducer; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 394 AA 
Protein Sequence
MGCLGNSKTE DQRNEEKAQR EANKKIEKQL QKDKQVYRAT HRLLLLGAGE SGKSTIVKQM 60
RILHVNGFNG EGGEEDPQAA RSNSDGEKAT KVQDIKNNLK EAIETIVAAM SNLVPPVELA 120
NPENQFRVDY ILSVMNVPDF DFPPEFYEHA KALWEDEGVR ACYERSNEYQ LIDCAQYFLD 180
KIDVIKQDDY VPSDQDLLRC RVLTSGIFET KFQVDKVNFH MFDVGGQRDE RRKWIQCFND 240
VTAIIFVVAS SSYNMVIRED NQTNRLQEAL NLFKSIWNNR WLRTISVILF LNKQDLLAEK 300
VLAGKSKIED YFPEFARYTT PEDATPEPGE DPRVTRAKYF IRDEFLRIST ASGDGRHYCY 360
PHFTCAVDTE NIRRVFNDCR DIIQRMHLRQ YELL 394 
Gene Ontology
 GO:0030425; C:dendrite; IEA:Compara.
 GO:0005834; C:heterotrimeric G-protein complex; ISS:RefGenome.
 GO:0031683; F:G-protein beta/gamma-subunit complex binding; ISS:RefGenome.
 GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
 GO:0003924; F:GTPase activity; ISS:RefGenome.
 GO:0035255; F:ionotropic glutamate receptor binding; ISS:RefGenome.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0031852; F:mu-type opioid receptor binding; ISS:RefGenome.
 GO:0004871; F:signal transducer activity; ISS:RefGenome.
 GO:0007191; P:adenylate cyclase-activating dopamine receptor signaling pathway; ISS:RefGenome.
 GO:0007610; P:behavior; IEA:Compara.
 GO:0051216; P:cartilage development; IEA:Compara.
 GO:0006306; P:DNA methylation; IEA:Compara.
 GO:0048701; P:embryonic cranial skeleton morphogenesis; IEA:Compara.
 GO:0035116; P:embryonic hindlimb morphogenesis; IEA:Compara.
 GO:0001958; P:endochondral ossification; IEA:Compara.
 GO:0006112; P:energy reserve metabolic process; IEA:Compara.
 GO:0071514; P:genetic imprinting; IEA:Compara.
 GO:0035264; P:multicellular organism growth; IEA:Compara.
 GO:0045669; P:positive regulation of osteoblast differentiation; IEA:Compara.
 GO:0045672; P:positive regulation of osteoclast differentiation; IEA:Compara.
 GO:0040032; P:post-embryonic body morphogenesis; IEA:Compara.
 GO:0042493; P:response to drug; IEA:Compara.
 GO:0007606; P:sensory perception of chemical stimulus; ISS:RefGenome.
 GO:0043588; P:skin development; IEA:Compara.
 GO:0001894; P:tissue homeostasis; IEA:Compara. 
Interpro
 IPR000367; Gprotein_alpha_S.
 IPR001019; Gprotein_alpha_su.
 IPR011025; GproteinA_insert.
 IPR027417; P-loop_NTPase. 
Pfam
 PF00503; G-alpha 
SMART
 SM00275; G_alpha 
PROSITE
  
PRINTS
 PR00318; GPROTEINA.
 PR00443; GPROTEINAS.