UniProt Accession

 ERG1_YEAST; P32476; D6VUW0 

Genbank Protein ID

 M64994; Z72960; BK006941 

Genbank Nucleotide ID

 AAA34592.1; CAA97201.1; DAA08271.1 

Protein Name

 Squalene monooxygenase 

Protein Synonyms/Alias

 Squalene epoxidase; SE 

Gene Name

 ERG1 

Gene Synonyms/Alias

 YGR175C 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
135	EDSTIHIKDYEDDER	acetylation	[1]
278	LCAYNSPKVPADIKS	ubiquitination	[2, 3]
284	PKVPADIKSWMIKDV	ubiquitination	[2, 3, 4, 5, 6]
289	DIKSWMIKDVQPFIP	ubiquitination	[2, 3]
311	DEAVSQGKFRAMPNS	ubiquitination	[2, 3, 5, 6, 7, 8]
360	HDVVLLIKKIGDLDF	ubiquitination	[2, 3, 8]
372	LDFSDREKVLDELLD	ubiquitination	[2]
414	DNLKALQKGCFKYFQ	ubiquitination	[3]
418	ALQKGCFKYFQRGGD	acetylation	[1]
418	ALQKGCFKYFQRGGD	ubiquitination	[3]
429	RGGDCVNKPVEFLSG	ubiquitination	[3]
440	FLSGVLPKPLQLTRV	ubiquitination	[3]

Reference

 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [2] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
 Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
 Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047]
 [3] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [4] A subset of membrane-associated proteins is ubiquitinated in response to mutations in the endoplasmic reticulum degradation machinery.
 Hitchcock AL, Auld K, Gygi SP, Silver PA.
 Proc Natl Acad Sci U S A. 2003 Oct 28;100(22):12735-40. [PMID: 14557538]
 [5] Computational identification of ubiquitylation sites from protein sequences.
 Tung CW, Ho SY.
 BMC Bioinformatics. 2008 Jul 15;9:310. [PMID: 18625080]
 [6] Identification, analysis, and prediction of protein ubiquitination sites.
 Radivojac P, Vacic V, Haynes C, Cocklin RR, Mohan A, Heyen JW, Goebl MG, Iakoucheva LM.
 Proteins. 2010 Feb 1;78(2):365-80. [PMID: 19722269]
 [7] A proteomics approach to understanding protein ubiquitination.
 Peng J, Schwartz D, Elias JE, Thoreen CC, Cheng D, Marsischky G, Roelofs J, Finley D, Gygi SP.
 Nat Biotechnol. 2003 Aug;21(8):921-6. [PMID: 12872131]
 [8] Systematic approach for validating the ubiquitinated proteome.
 Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
 Anal Chem. 2008 Jun 1;80(11):4161-9. [PMID: 18433149] 

Functional Description

 Catalyzes the first oxygenation step in sterol biosynthesis and is suggested to be one of the rate-limiting enzymes in this pathway. 

Sequence Annotation

 NP_BIND 21 48 FAD (Potential).
 CROSSLNK 284 284 Glycyl lysine isopeptide (Lys-Gly)
 CROSSLNK 311 311 Glycyl lysine isopeptide (Lys-Gly)  

Keyword

 Complete proteome; Endoplasmic reticulum; FAD; Flavoprotein; Isopeptide bond; Membrane; Microsome; NADP; Oxidoreductase; Reference proteome; Transmembrane; Transmembrane helix; Ubl conjugation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 496 AA 

Protein Sequence

Gene Ontology

 GO:0005783; C:endoplasmic reticulum; IDA:SGD.
 GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0005811; C:lipid particle; IDA:SGD.
 GO:0008144; F:drug binding; IMP:SGD.
 GO:0050660; F:flavin adenine dinucleotide binding; IEA:InterPro.
 GO:0004506; F:squalene monooxygenase activity; IDA:SGD.
 GO:0006696; P:ergosterol biosynthetic process; IMP:SGD. 

Interpro

 IPR003042; Rng_hydrolase-like.
 IPR013698; Squalene_epoxidase. 

Pfam

 PF08491; SE 

SMART

  

PROSITE

  

PRINTS

 PR00420; RNGMNOXGNASE.