CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-011084
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Serine/threonine-protein kinase STE20 
Protein Synonyms/Alias
  
Gene Name
 STE20 
Gene Synonyms/Alias
 YHL007C 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
577TVTTPTSKPAQARSLubiquitination[1]
Reference
 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 
Functional Description
 MAP4K component of the MAPK pathway required for the mating pheromone response, haploid invasive growth and diploid pseudohyphal development. Links the pheromone response G-protein beta gamma subunits to downstream signaling components. Needed for mating in haploid cells, induction of a mating-specific gene FUS1, induction of mating-specific morphologies, and pheromone-induced proliferation arrest. Required for the regulation of the actin polarization and bud emergence during cell cycle in G1. Involved in the high osmolarity glycerol (HOG) response. Phosphorylates 'Thr-307' and 'Ser-302' or 'Ser-306' of STE11 and 'Ser-357' of MYO3. Phosphorylates histone H2B to form H2BS10ph during meiosis and H(2)O(2)-induced apoptosis. Its interaction with CDC42 is required for both invasive growth and the formation of pseudohyphae. Its interaction with STE4 is required for the pheromone signaling. 
Sequence Annotation
 DOMAIN 337 350 CRIB.
 DOMAIN 620 871 Protein kinase.
 NP_BIND 626 634 ATP (By similarity).
 REGION 434 499 BEM1-binding.
 ACT_SITE 739 739 Proton acceptor (By similarity).
 BINDING 649 649 ATP (By similarity).
 MOD_RES 87 87 Phosphoserine.
 MOD_RES 165 165 Phosphoserine.
 MOD_RES 167 167 Phosphothreonine.
 MOD_RES 169 169 Phosphoserine.
 MOD_RES 203 203 Phosphothreonine.
 MOD_RES 418 418 Phosphoserine.
 MOD_RES 502 502 Phosphoserine.
 MOD_RES 547 547 Phosphoserine.
 MOD_RES 562 562 Phosphoserine.
 MOD_RES 573 573 Phosphothreonine.
 MOD_RES 585 585 Phosphoserine.
 MOD_RES 773 773 Phosphothreonine.
 MOD_RES 924 924 Phosphoserine.
 MOD_RES 927 927 Phosphothreonine.  
Keyword
 3D-structure; ATP-binding; Cell cycle; Complete proteome; Cytoplasm; Direct protein sequencing; Kinase; Nucleotide-binding; Nucleus; Pheromone response; Phosphoprotein; Reference proteome; Serine/threonine-protein kinase; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 939 AA 
Protein Sequence
MSNDPSAVSE LPDKDSLDNG ISNDNERAMG GNGDGGDGLR LPRTTGTLNV NALQKGTNAA 60
HEAGGYKSMD PAKNAETTND DDNNVVSLDD PIQFTRVSSS SVISGMSSSM SPHSNIDETK 120
SLEAVTPNIN TSNITPDHSA DNTFSTINAS ESDHQFNDTL LSKLSLTDST ETIENNATVK 180
HQQPVASSTV NSNKSSTDIR RATPVSTPVI SKPSMTTTPR QINSASHSLS NPKHKQHKPK 240
VKPSKPEAKS KPVSVKKSFP SKNPLKNSSP PKKQTEKSYY SSSSKKRKSG SNSGTLRMKD 300
VFTSFVQNIK RNSQDDKRAS SSSNNSSSSS ITTALRISTP YNAKHIHHVG VDSKTGEYTG 360
LPEEWEKLLT SSGISKREQQ QNMQAVMDIV KFYQDVTETN GEDKMFKTFN TTTGLPGSPQ 420
VSTPPANSFN KFPPSTSDSH NYGSRTGTPM SNHVMSPTLN TDSSSANGKF IPSRPAPKPP 480
SSASASAPII KSPVMNSAAN VSPLKQTHAP TTPNRTSPNR SSISRNATLK KEEQPLPPIP 540
PTKSKTSPII STAHTPQQVA QSPKAPAQET VTTPTSKPAQ ARSLSKELNE KKREERERRK 600
KQLYAKLNEI CSDGDPSTKY ANLVKIGQGA SGGVYTAYEI GTNVSVAIKQ MNLEKQPKKE 660
LIINEILVMK GSKHPNIVNF IDSYVLKGDL WVIMEYMEGG SLTDVVTHCI LTEGQIGAVC 720
RETLSGLEFL HSKGVLHRDI KSDNILLSME GDIKLTDFGF CAQINELNLK RTTMVGTPYW 780
MAPEVVSRKE YGPKVDIWSL GIMIIEMIEG EPPYLNETPL RALYLIATNG TPKLKEPENL 840
SSSLKKFLDW CLCVEPEDRA SATELLHDEY ITEIAEANSS LAPLVKLARL KKVAENMDAD 900
EDNDDDNDNE HINKTNNCDD NNDSKETVNL DVTEDDKQK 939 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0000131; C:incipient cellular bud site; IDA:SGD.
 GO:0043332; C:mating projection tip; IDA:SGD.
 GO:0005634; C:nucleus; IDA:SGD.
 GO:0005886; C:plasma membrane; IDA:SGD.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0035174; F:histone serine kinase activity; IDA:SGD.
 GO:0008349; F:MAP kinase kinase kinase kinase activity; IDA:SGD.
 GO:0000282; P:cellular bud site selection; IMP:SGD.
 GO:0034605; P:cellular response to heat; IMP:SGD.
 GO:0001403; P:invasive growth in response to glucose limitation; IMP:SGD.
 GO:0010629; P:negative regulation of gene expression; IMP:SGD.
 GO:0007232; P:osmosensory signaling pathway via Sho1 osmosensor; IMP:SGD.
 GO:0000750; P:pheromone-dependent signal transduction involved in conjugation with cellular fusion; IMP:SGD.
 GO:0043065; P:positive regulation of apoptotic process; IMP:SGD.
 GO:0007124; P:pseudohyphal growth; IMP:SGD.
 GO:0007096; P:regulation of exit from mitosis; IMP:SGD.
 GO:0001402; P:signal transduction involved in filamentous growth; IMP:SGD.
 GO:0035376; P:sterol import; IMP:SGD.
 GO:0034063; P:stress granule assembly; IMP:SGD.
 GO:0000011; P:vacuole inheritance; IMP:SGD. 
Interpro
 IPR011009; Kinase-like_dom.
 IPR000095; PAK_box_Rho-bd.
 IPR000719; Prot_kinase_cat_dom.
 IPR017441; Protein_kinase_ATP_BS.
 IPR002290; Ser/Thr_dual-sp_kinase_dom.
 IPR008271; Ser/Thr_kinase_AS. 
Pfam
 PF00786; PBD
 PF00069; Pkinase 
SMART
 SM00285; PBD
 SM00220; S_TKc 
PROSITE
 PS50108; CRIB
 PS00107; PROTEIN_KINASE_ATP
 PS50011; PROTEIN_KINASE_DOM
 PS00108; PROTEIN_KINASE_ST 
PRINTS