CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-024115
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 RAS guanyl-releasing protein 1 
Protein Synonyms/Alias
 Calcium and DAG-regulated guanine nucleotide exchange factor II; CalDAG-GEFII; Ras guanyl-releasing protein 
Gene Name
 Rasgrp1 
Gene Synonyms/Alias
 Rasgrp 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
773QLKYAQKKIESLQLGubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 
Functional Description
 Functions as a diacylglycerol (DAG)-regulated nucleotide exchange factor specifically activating Ras through the exchange of bound GDP for GTP. Activates the Erk/MAP kinase cascade. Couples T-lymphocytes and B-lymphocytes antigen receptors to the activation of Ras. Hence, regulates T-cells and B-cells development, homeostasis and differentiation. Functions also in FcERI-evoked degranulation and cytokine secretion by mast cells, regulating allergic responses. May also function in other cell types differentiation. Proto-oncogene which promotes T-cell lymphomagenesis when its expression is deregulated. 
Sequence Annotation
 DOMAIN 53 176 N-terminal Ras-GEF.
 DOMAIN 205 436 Ras-GEF.
 DOMAIN 470 505 EF-hand 1.
 DOMAIN 497 532 EF-hand 2.
 ZN_FING 541 591 Phorbol-ester/DAG-type.
 REGION 57 110 Ras exchanger motif region; required for
 REGION 686 694 Suppress the PT region-mediated
 REGION 717 795 PT region; mediates the BCR-dependent
 MOD_RES 184 184 Phosphothreonine; by PKC (By similarity).  
Keyword
 Calcium; Cell membrane; Coiled coil; Complete proteome; Cytoplasm; Differentiation; Endoplasmic reticulum; Golgi apparatus; Guanine-nucleotide releasing factor; Membrane; Metal-binding; Phosphoprotein; Proto-oncogene; Reference proteome; Repeat; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 795 AA 
Protein Sequence
MGTLGKAREA PRKPCHGSRA GPKARLEAKS TNSPLPAQPS LAQITQFRMM VSLGHLAKGA 60
SLDDLIDSCI QSFDADGNLC RNNQLLQVML TMHRIIISSA ELLQKVMNLY KDALEKNSPG 120
VCLKICYFVR YWITEFWIMF KMDASLTSTM EEFQDLVKAN GEETHCHLID TTQINSRDWS 180
RKLTQRIKSN TSKKRKVSLL FDHLEPEELS EHLTYLEFKS FRRISFSDYQ NYLVNSCVKE 240
NPTMERSIAL CNGISQWVQL MVLSRPTPQL RAEVFIKFIH VAQKLHQLQN FNTLMAVIGG 300
LCHSSISRLK ETSSHVPHEI NKVLGEMTEL LSSCRNYDNY RRAYGECTHF KIPILGVHLK 360
DLISLYEAMP DYLEDGKVNV QKLLALYNHI NELVQLQEMA PPLDANKDLV HLLTLSLDLY 420
YTEDEIYELS YAREPRNHRA PPLTPSKPPV VVDWASGVSP KPDPKTISKH VQRMVDSVFK 480
NYDLDQDGYI SQEEFEKIAA SFPFSFCVMD KDREGLISRD EITAYFMRAS SIYSKLGLGF 540
PHNFQETTYL KPTFCDNCAG FLWGVIKQGY RCKDCGMNCH KQCKDLVVFE CKKRIKSPAI 600
STENISSVVP MSTLCPLGTK DLLHAPEEGS FIFQNGEIVD HSEESKDRTI MLLGVSSQKI 660
SVRLKRTVAH KSTQTESFPW VGGETTPGHF VLSSPRKSAQ GALYVHSPAS PCPSPALVRK 720
RAFVKWENKE SLIKPKPELH LRLRTYQELE QEINTLKADN DALKIQLKYA QKKIESLQLG 780
KSNHVLAQMD HGDSA 795 
Gene Ontology
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
 GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
 GO:0005886; C:plasma membrane; TAS:Reactome.
 GO:0005509; F:calcium ion binding; IEA:InterPro.
 GO:0005085; F:guanyl-nucleotide exchange factor activity; IEA:UniProtKB-KW.
 GO:0032862; P:activation of Rho GTPase activity; IMP:MGI.
 GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
 GO:0001816; P:cytokine production; IMP:MGI.
 GO:0002437; P:inflammatory response to antigenic stimulus; IMP:MGI.
 GO:0043303; P:mast cell degranulation; IMP:MGI.
 GO:0014066; P:regulation of phosphatidylinositol 3-kinase cascade; IMP:MGI.
 GO:0032252; P:secretory granule localization; IMP:MGI.
 GO:0007264; P:small GTPase mediated signal transduction; IEA:InterPro.
 GO:0047496; P:vesicle transport along microtubule; IMP:MGI. 
Interpro
 IPR020454; DAG/PE-bd.
 IPR011992; EF-hand-like_dom.
 IPR018247; EF_Hand_1_Ca_BS.
 IPR002048; EF_hand_dom.
 IPR002219; Prot_Kinase_C-like_PE/DAG-bd.
 IPR000651; Ras-like_Gua-exchang_fac_N.
 IPR008937; Ras_GEF.
 IPR023578; Ras_GEF_dom.
 IPR001895; RasGRF_CDC25. 
Pfam
 PF00130; C1_1
 PF13499; EF_hand_5
 PF00617; RasGEF
 PF00618; RasGEF_N 
SMART
 SM00109; C1
 SM00147; RasGEF
 SM00229; RasGEFN 
PROSITE
 PS00018; EF_HAND_1
 PS50222; EF_HAND_2
 PS00720; RASGEF
 PS50009; RASGEF_CAT
 PS50212; RASGEF_NTER
 PS00479; ZF_DAG_PE_1
 PS50081; ZF_DAG_PE_2 
PRINTS
 PR00008; DAGPEDOMAIN.