UniProt Accession

 CPT1_YEAST; P17898; D6W152 

Genbank Protein ID

 J05203; Z46843; Z71406; BK006947 

Genbank Nucleotide ID

 AAA63571.1; CAA86895.1; CAA96012.1; DAA10418.1 

Protein Name

 Cholinephosphotransferase 1 

Protein Synonyms/Alias

 Aminoalcohol phosphotransferase CPT1; Diacylglycerol cholinephosphotransferase 1; Sn-1,2-diacylglycerol cholinephosphotransferase; CHOPT 

Gene Name

 CPT1 

Gene Synonyms/Alias

 YNL130C; N1218; N1867 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
17	LGNLKLYKYQSDDRS	acetylation	[1]
17	LGNLKLYKYQSDDRS	ubiquitination	[2]
250	YYESQSTKSATPSKT	ubiquitination	[2]
256	TKSATPSKTAENISK	ubiquitination	[2]

Reference

 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [2] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 

Functional Description

 Catalyzes the final step in the CDP-choline route leading to phosphatidylcholin (PC). Preferentially uses CDP- monomethylethanolamine as aminoalcohol substrate. Shows highest activity toward di- and mono-unsaturated diacylglycerol species as lipid substrates. The CDP-choline pathway only contributes to net PC synthesis if exogenous choline is present. In its absence, this pathway recycles choline from PC turnover and may contribute to maintaining the proper PC species composition. 

Sequence Annotation

  

Keyword

 Complete proteome; Endoplasmic reticulum; Lipid biosynthesis; Lipid metabolism; Magnesium; Membrane; Metal-binding; Microsome; Mitochondrion; Mitochondrion outer membrane; Phospholipid biosynthesis; Phospholipid metabolism; Reference proteome; Transferase; Transmembrane; Transmembrane helix. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 393 AA 

Protein Sequence

Gene Ontology

 GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
 GO:0005794; C:Golgi apparatus; IDA:SGD.
 GO:0016021; C:integral to membrane; ISM:SGD.
 GO:0005741; C:mitochondrial outer membrane; IDA:SGD.
 GO:0004142; F:diacylglycerol cholinephosphotransferase activity; IDA:SGD.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW. 

Interpro

 IPR000462; CDP-OH_P_trans.
 IPR014472; CHOPT. 

Pfam

 PF01066; CDP-OH_P_transf 

SMART

  

PROSITE

 PS00379; CDP_ALCOHOL_P_TRANSF 

PRINTS