UniProt Accession

 NFL_MOUSE; P08551; Q8K0Z0 

Genbank Protein ID

 M20480; M13016; DQ201635; BC029203; AH002053; X02165 

Genbank Nucleotide ID

 AAA39814.1; AAA39810.1; ABA46748.1; AAH29203.1; AAA39812.1; CAB51616.1 

Protein Name

 Neurofilament light polypeptide 

Protein Synonyms/Alias

 NF-L; 68 kDa neurofilament protein; Neurofilament triplet L protein 

Gene Name

 Nefl 

Gene Synonyms/Alias

 Nf68; Nfl 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
15	PYFSTSYKRRYVETP	ubiquitination	[1]
268	DIRAQYEKLAAKNMQ	ubiquitination	[1]
272	QYEKLAAKNMQNAEE	ubiquitination	[1]
282	QNAEEWFKSRFTVLT	ubiquitination	[1]
294	VLTESAAKNTDAVRA	ubiquitination	[1]
316	SRRLLKAKTLEIEAC	ubiquitination	[1]
332	GMNEALEKQLQELED	ubiquitination	[1]
371	SEMARYLKEYQDLLN	ubiquitination	[1]
392	IEIAAYRKLLEGEET	ubiquitination	[1]

Reference

 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 

Functional Description

 Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. 

Sequence Annotation

 REGION 2 93 Head.
 REGION 94 397 Rod.
 REGION 94 125 Coil 1A.
 REGION 126 138 Linker 1.
 REGION 139 234 Coil 1B.
 REGION 235 253 Linker 12.
 REGION 254 272 Coil 2A.
 REGION 273 281 Linker 2.
 REGION 282 397 Coil 2B.
 REGION 382 392 Epitope; recognized by IF-specific
 REGION 398 543 Tail.
 REGION 398 444 Tail, subdomain A.
 REGION 445 543 Tail, subdomain B (acidic).
 MOD_RES 2 2 N-acetylserine (By similarity).
 MOD_RES 43 43 Phosphotyrosine.
 MOD_RES 56 56 Phosphoserine.
 MOD_RES 67 67 Phosphoserine (By similarity).
 MOD_RES 425 425 Phosphotyrosine.
 MOD_RES 433 433 Phosphotyrosine.
 MOD_RES 473 473 Phosphoserine.
 MOD_RES 532 532 Phosphoserine.
 CARBOHYD 21 21 O-linked (GlcNAc) (By similarity).
 CARBOHYD 27 27 O-linked (GlcNAc) (By similarity).  

Keyword

 Acetylation; Coiled coil; Complete proteome; Direct protein sequencing; Glycoprotein; Intermediate filament; Phosphoprotein; Reference proteome; Ubl conjugation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 543 AA 

Protein Sequence

Gene Ontology

 GO:0030426; C:growth cone; IEA:Compara.
 GO:0005883; C:neurofilament; IDA:MGI.
 GO:0005198; F:structural molecule activity; IEA:InterPro.
 GO:0045110; P:intermediate filament bundle assembly; IGI:MGI.
 GO:0040011; P:locomotion; IMP:MGI.
 GO:0000226; P:microtubule cytoskeleton organization; IMP:MGI.
 GO:0043524; P:negative regulation of neuron apoptotic process; IMP:MGI.
 GO:0060052; P:neurofilament cytoskeleton organization; IMP:MGI.
 GO:0050885; P:neuromuscular process controlling balance; IMP:MGI.
 GO:0014012; P:peripheral nervous system axon regeneration; IMP:MGI.
 GO:0050772; P:positive regulation of axonogenesis; IMP:MGI.
 GO:0051258; P:protein polymerization; IEA:Compara.
 GO:0031133; P:regulation of axon diameter; IMP:MGI.
 GO:0051412; P:response to corticosterone stimulus; IEA:Compara.
 GO:0043434; P:response to peptide hormone stimulus; IEA:Compara.
 GO:0009636; P:response to toxic substance; IEA:Compara. 

Interpro

 IPR016044; F.
 IPR001664; IF.
 IPR006821; Intermed_filament_DNA-bd.
 IPR018039; Intermediate_filament_CS. 

Pfam

 PF00038; Filament
 PF04732; Filament_head 

SMART

  

PROSITE

 PS00226; IF 

PRINTS