CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-014364
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Protein kinase C and casein kinase substrate in neurons protein 1 
Protein Synonyms/Alias
 Syndapin 1 
Gene Name
 Pacsin1 
Gene Synonyms/Alias
 Pacsin 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
51QERAKIEKAYAQQLTubiquitination[1]
70RWRQLIEKGPQYGSLubiquitination[1]
99SELHQEVKNSLLNEDubiquitination[1]
120WQKDAYHKQIMGGFKubiquitination[1]
250KEVLLDIKRHLNLAEubiquitination[1]
319KKEKQPKKAEGATLSubiquitination[1]
411KAGDELTKLGEEDEQubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 
Functional Description
 Binds to membranes via its F-BAR domain and mediates membrane tubulation. Plays a role in the reorganization of the microtubule cytoskeleton via its interaction with MAPT; this decreases microtubule stability and inhibits MAPT-induced microtubule polymerization. Plays a role in cellular transport processes by recruiting DNM1, DNM2 and DNM3 to membranes. Plays a role in the reorganization of the actin cytoskeleton and in neuron morphogenesis via its interaction with COBL and WASL, and by recruiting COBL to the cell cortex. Plays a role in the regulation of neurite formation, neurite branching and the regulation of neurite length. Required for normal synaptic vesicle endocytosis; this process retrieves previously released neurotransmitters to accomodate multiple cycles of neurotransmission. Required for normal excitatory and inhibitory synaptic transmission. 
Sequence Annotation
 DOMAIN 10 73 FCH.
 DOMAIN 382 441 SH3.
 REGION 1 304 F-BAR domain.
 MOD_RES 76 76 Phosphoserine (By similarity).
 MOD_RES 181 181 Phosphothreonine (By similarity).
 MOD_RES 391 391 Phosphotyrosine.  
Keyword
 3D-structure; Cell junction; Cell membrane; Cell projection; Coiled coil; Complete proteome; Cytoplasm; Cytoplasmic vesicle; Direct protein sequencing; Endocytosis; Lipid-binding; Membrane; Phosphoprotein; Reference proteome; SH3 domain; Synapse; Synaptosome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 441 AA 
Protein Sequence
MSGSYDEASE EITDSFWEVG NYKRTVKRID DGHRLCNDLM SCVQERAKIE KAYAQQLTDW 60
AKRWRQLIEK GPQYGSLERA WGAMMTEADK VSELHQEVKN SLLNEDLEKV KNWQKDAYHK 120
QIMGGFKETK EAEDGFRKAQ KPWAKKMKEL EAAKKAYHLA CKEERLAMTR EMNSKTEQSV 180
TPEQQKKLVD KVDKCRQDVQ KTQEKYEKVL EDVGKTTPQY MEGMEQVFEQ CQQFEEKRLV 240
FLKEVLLDIK RHLNLAENSS YMHVYRELEQ AIRGADAQED LRWFRSTSGP GMPMNWPQFE 300
EWNPDLPHTT AKKEKQPKKA EGATLSNATG AVESTSQAGD RGSVSSYDRG QTYATEWSDD 360
ESGNPFGGNE ANGGANPFED DAKGVRVRAL YDYDGQEQDE LSFKAGDELT KLGEEDEQGW 420
CRGRLDSGQL GLYPANYVEA I 441 
Gene Ontology
 GO:0043679; C:axon terminus; ISS:UniProtKB.
 GO:0030054; C:cell junction; IEA:UniProtKB-KW.
 GO:0030137; C:COPI-coated vesicle; IDA:MGI.
 GO:0030659; C:cytoplasmic vesicle membrane; IEA:UniProtKB-SubCell.
 GO:0005829; C:cytosol; IEA:UniProtKB-SubCell.
 GO:0032587; C:ruffle membrane; IDA:UniProtKB.
 GO:0045202; C:synapse; IEA:UniProtKB-SubCell.
 GO:0008092; F:cytoskeletal protein binding; IDA:MGI.
 GO:0005543; F:phospholipid binding; IDA:UniProtKB.
 GO:0097320; P:membrane tubulation; IMP:UniProtKB.
 GO:0045806; P:negative regulation of endocytosis; IDA:MGI.
 GO:0048812; P:neuron projection morphogenesis; ISS:UniProtKB.
 GO:0072657; P:protein localization to membrane; IMP:UniProtKB.
 GO:0007165; P:signal transduction; TAS:MGI.
 GO:0048488; P:synaptic vesicle endocytosis; IMP:UniProtKB. 
Interpro
 IPR001060; FCH_dom.
 IPR001452; SH3_domain. 
Pfam
 PF00611; FCH
 PF00018; SH3_1 
SMART
 SM00055; FCH
 SM00326; SH3 
PROSITE
 PS50133; FCH
 PS50002; SH3 
PRINTS
 PR00452; SH3DOMAIN.