CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-002160
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Threonine--tRNA ligase, cytoplasmic 
Protein Synonyms/Alias
 Threonyl-tRNA synthetase; ThrRS 
Gene Name
 THS1 
Gene Synonyms/Alias
 YIL078W 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
76SMPRVPLKIVLKDGAacetylation[1]
80VPLKIVLKDGAVKEAacetylation[1]
103DIAKGISKSLADRLCacetylation[1]
213PGLEGVAKNVIKQKQacetylation[1]
229FERLVMSKEDLLKMFacetylation[1]
234MSKEDLLKMFHYSKYacetylation[1]
240LKMFHYSKYKTYLVQacetylation[1]
242MFHYSKYKTYLVQTKacetylation[1]
249KTYLVQTKVPDGGATacetylation[1]
249KTYLVQTKVPDGGATubiquitination[2]
286IKAFKLLKNSSCYFLubiquitination[2]
312YGISFPDKKLMDAHLacetylation[1]
515RPEKYVGKIETWDAAacetylation[1]
515RPEKYVGKIETWDAAubiquitination[2]
525TWDAAESKLESALKKacetylation[1]
638LVVPVGVKYQGYAEDacetylation[1]
718VSVEEVLKQLRNLKDacetylation[1]
Reference
 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [2] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 
Functional Description
  
Sequence Annotation
 MOD_RES 195 195 Phosphoserine.
 MOD_RES 289 289 Phosphoserine.
 MOD_RES 297 297 Phosphothreonine.
 MOD_RES 381 381 Phosphothreonine.
 MOD_RES 453 453 Phosphoserine.
 MOD_RES 457 457 Phosphoserine.
 MOD_RES 460 460 Phosphothreonine.
 MOD_RES 605 605 Phosphoserine.  
Keyword
 Aminoacyl-tRNA synthetase; ATP-binding; Complete proteome; Cytoplasm; Ligase; Nucleotide-binding; Phosphoprotein; Protein biosynthesis; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 734 AA 
Protein Sequence
MSASEAGVTE QVKKLSVKDS SNDAVKPNKK ENKKSKQQSL YLDPEPTFIE ERIEMFDRLQ 60
KEYNDKVASM PRVPLKIVLK DGAVKEATSW ETTPMDIAKG ISKSLADRLC ISKVNGQLWD 120
LDRPFEGEAN EEIKLELLDF ESDEGKKVFW HSSAHVLGES CECHLGAHIC LGPPTDDGFF 180
YEMAVRDSMK DISESPERTV SQADFPGLEG VAKNVIKQKQ KFERLVMSKE DLLKMFHYSK 240
YKTYLVQTKV PDGGATTVYR CGKLIDLCVG PHIPHTGRIK AFKLLKNSSC YFLGDATNDS 300
LQRVYGISFP DKKLMDAHLK FLAEASMRDH RKIGKEQELF LFNEMSPGSC FWLPHGTRIY 360
NTLVDLLRTE YRKRGYEEVI TPNMYNSKLW ETSGHWANYK ENMFTFEVEK ETFGLKPMNC 420
PGHCLMFKSR ERSYRELPWR VADFGVIHRN EFSGALSGLT RVRRFQQDDA HIFCTHDQIE 480
SEIENIFNFL QYIYGVFGFE FKMELSTRPE KYVGKIETWD AAESKLESAL KKWGGNWEIN 540
AGDGAFYGPK IDIMISDALR RWHQCATIQL DFQLPNRFEL EFKSKDQDSE SYERPVMIHR 600
AILGSVERMT AILTEHFAGK WPFWLSPRQV LVVPVGVKYQ GYAEDVRNKL HDAGFYADVD 660
LTGNTLQKKV RNGQMLKYNF IFIVGEQEMN EKSVNIRNRD VMEQQGKNAT VSVEEVLKQL 720
RNLKDEKRGD NVLA 734 
Gene Ontology
 GO:0005739; C:mitochondrion; IDA:SGD.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004829; F:threonine-tRNA ligase activity; IDA:SGD.
 GO:0002181; P:cytoplasmic translation; IMP:SGD.
 GO:0006435; P:threonyl-tRNA aminoacylation; IDA:SGD. 
Interpro
 IPR002314; aa-tRNA-synt_IIb_cons-dom.
 IPR006195; aa-tRNA-synth_II.
 IPR004154; Anticodon-bd.
 IPR012675; Beta-grasp_dom.
 IPR004095; TGS.
 IPR012676; TGS-like.
 IPR002320; Thr-tRNA-ligase_IIa.
 IPR018163; Thr/Ala-tRNA-synth_IIc_edit.
 IPR012947; tRNA_SAD. 
Pfam
 PF03129; HGTP_anticodon
 PF02824; TGS
 PF00587; tRNA-synt_2b
 PF07973; tRNA_SAD 
SMART
 SM00863; tRNA_SAD 
PROSITE
 PS50862; AA_TRNA_LIGASE_II 
PRINTS
 PR01047; TRNASYNTHTHR.