CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-019000
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 MKL/myocardin-like protein 1 
Protein Synonyms/Alias
 Megakaryoblastic leukemia 1 protein; Megakaryocytic acute leukemia protein; Myocardin-related transcription factor A; MRTF-A 
Gene Name
 MKL1 
Gene Synonyms/Alias
 KIAA1438; MAL 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
499SPLQILVKEEGPRAGsumoylation[1]
576APLGTPVKQENSFSSsumoylation[1]
624GPPSVVVKQEALQPEsumoylation[1]
Reference
 [1] Transcriptional activity of megakaryoblastic leukemia 1 (MKL1) is repressed by SUMO modification.
 Nakagawa K, Kuzumaki N.
 Genes Cells. 2005 Aug;10(8):835-50. [PMID: 16098147] 
Functional Description
 Transcriptional coactivator of serum response factor (SRF) with the potential to modulate SRF target genes. Suppresses TNF-induced cell death by inhibiting activation of caspases; its transcriptional activity is indispensable for the antiapoptotic function. It may up-regulate antiapoptotic molecules, which in turn inhibit caspase activation (By similarity). 
Sequence Annotation
 REPEAT 24 49 RPEL 1.
 REPEAT 68 93 RPEL 2.
 DOMAIN 347 381 SAP.
 REGION 1 256 Mediates interaction with SCAI and ACTB
 MOD_RES 6 6 Phosphoserine.
 MOD_RES 305 305 Phosphothreonine.
 MOD_RES 450 450 Phosphothreonine.
 MOD_RES 454 454 Phosphoserine.  
Keyword
 3D-structure; Actin-binding; Chromosomal rearrangement; Coiled coil; Complete proteome; Cytoplasm; Nucleus; Phosphoprotein; Polymorphism; Proto-oncogene; Reference proteome; Repeat; Transcription; Transcription regulation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 931 AA 
Protein Sequence
MPPLKSPAAF HEQRRSLERA RTEDYLKRKI RSRPERSELV RMHILEETSA EPSLQAKQLK 60
LKRARLADDL NEKIAQRPGP MELVEKNILP VESSLKEAII VGQVNYPKVA DSSSFDEDSS 120
DALSPEQPAS HESQGSVPSP LEARVSEPLL SATSASPTQV VSQLPMGRDS REMLFLAEQP 180
PLPPPPLLPP SLTNGTTIPT AKSTPTLIKQ SQPKSASEKS QRSKKAKELK PKVKKLKYHQ 240
YIPPDQKQDR GAPPMDSSYA KILQQQQLFL QLQILNQQQQ QHHNYQAILP APPKSAGEAL 300
GSSGTPPVRS LSTTNSSSSS GAPGPCGLAR QNSTSLTGKP GALPANLDDM KVAELKQELK 360
LRSLPVSGTK TELIERLRAY QDQISPVPGA PKAPAATSIL HKAGEVVVAF PAARLSTGPA 420
LVAAGLAPAE VVVATVASSG VVKFGSTGST PPVSPTPSER SLLSTGDENS TPGDTFGEMV 480
TSPLTQLTLQ ASPLQILVKE EGPRAGSCCL SPGGRAELEG RDKDQMLQEK DKQIEALTRM 540
LRQKQQLVER LKLQLEQEKR AQQPAPAPAP LGTPVKQENS FSSCQLSQQP LGPAHPFNPS 600
LAAPATNHID PCAVAPGPPS VVVKQEALQP EPEPVPAPQL LLGPQGPSLI KGVAPPTLIT 660
DSTGTHLVLT VTNKNADSPG LSSGSPQQPS SQPGSPAPAP SAQMDLEHPL QPLFGTPTSL 720
LKKEPPGYEE AMSQQPKQQE NGSSSQQMDD LFDILIQSGE ISADFKEPPS LPGKEKPSPK 780
TVCGSPLAAQ PSPSAELPQA APPPPGSPSL PGRLEDFLES STGLPLLTSG HDGPEPLSLI 840
DDLHSQMLSS TAILDHPPSP MDTSELHFVP EPSSTMGLDL ADGHLDSMDW LELSSGGPVL 900
SLAPLSTTAP SLFSTDFLDG HDLQLHWDSC L 931 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:BHF-UCL.
 GO:0005634; C:nucleus; IDA:BHF-UCL.
 GO:0003785; F:actin monomer binding; ISS:UniProtKB.
 GO:0003700; F:sequence-specific DNA binding transcription factor activity; IEA:Compara.
 GO:0003713; F:transcription coactivator activity; ISS:UniProtKB.
 GO:0000976; F:transcription regulatory region sequence-specific DNA binding; IEA:Compara.
 GO:0043066; P:negative regulation of apoptotic process; IEA:Compara.
 GO:0045944; P:positive regulation of transcription from RNA polymerase II promoter; IDA:BHF-UCL.
 GO:0051145; P:smooth muscle cell differentiation; IMP:BHF-UCL.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 
Interpro
 IPR004018; RPEL_repeat.
 IPR003034; SAP_dom. 
Pfam
 PF02755; RPEL
 PF02037; SAP 
SMART
 SM00707; RPEL
 SM00513; SAP 
PROSITE
 PS51073; RPEL
 PS50800; SAP 
PRINTS