CPLM-019882

Genbank Nucleotide ID

Alpha-aminoadipic semialdehyde synthase, mitochondrial

Protein Synonyms/Alias

LKR/SDH; Lysine ketoglutarate reductase; LKR; LOR; Saccharopine dehydrogenase; SDH

Mus musculus (Mouse)

Position	Peptide	Type	References
23	LSRGLHHKPVMALRR	acetylation	[1]
45	RRAPLAPKHIKGITK	acetylation	[2, 3]
48	PLAPKHIKGITKLGY	acetylation	[1, 2, 3]
52	KHIKGITKLGYKVLI	acetylation	[1, 2, 3, 4, 5]
52	KHIKGITKLGYKVLI	succinylation	[5]
56	GITKLGYKVLIQPSN	acetylation	[1, 2, 3, 5]
56	GITKLGYKVLIQPSN	succinylation	[5]
70	NRRAIHDKEYVRAGG	acetylation	[1, 2, 3, 5]
70	NRRAIHDKEYVRAGG	succinylation	[5]
93	ACLILGVKRPPEEKL	acetylation	[1, 5]
93	ACLILGVKRPPEEKL	succinylation	[5]
99	VKRPPEEKLMSKKTY	acetylation	[2, 3]
104	EEKLMSKKTYAFFSH	acetylation	[1]
128	NLLDEVLKQEIRLID	acetylation	[1, 2, 3]
128	NLLDEVLKQEIRLID	ubiquitination	[6]
138	IRLIDYEKMVDHRGS	acetylation	[1, 2, 3, 4, 5, 7, 8]
138	IRLIDYEKMVDHRGS	succinylation	[5]
253	HELREVSKTGDLRKV	acetylation	[2, 3, 5]
253	HELREVSKTGDLRKV	succinylation	[5]
259	SKTGDLRKVYGTVLS	acetylation	[1]
259	SKTGDLRKVYGTVLS	ubiquitination	[6]
274	RHHHLVRKTDGVYDP	acetylation	[2, 5]
274	RHHHLVRKTDGVYDP	succinylation	[5]
286	YDPVEYEKYPERYTS	acetylation	[1, 2, 3, 4, 5, 7, 8]
286	YDPVEYEKYPERYTS	succinylation	[5]
333	QSLLVPVKSSVVPVE	acetylation	[5]
333	QSLLVPVKSSVVPVE	succinylation	[5]
458	SNGLLTDKYKYIQKL	acetylation	[1, 2, 3, 4, 5, 7]
458	SNGLLTDKYKYIQKL	succinylation	[5]
458	SNGLLTDKYKYIQKL	ubiquitination	[6]
460	GLLTDKYKYIQKLRE	acetylation	[1, 2, 3]
464	DKYKYIQKLRESRER	acetylation	[2]
523	QMQQLSKKYNINPVS	acetylation	[2]
535	PVSLTVGKQEAKLQS	acetylation	[2]
584	SYITPAMKELEKSVD	acetylation	[2]
707	YPNRDSIKYAEIYGI	acetylation	[1, 2, 3, 4, 5]
707	YPNRDSIKYAEIYGI	succinylation	[5]
707	YPNRDSIKYAEIYGI	ubiquitination	[6]
732	LRYKGYSKALNGFVK	acetylation	[2, 3, 5]
732	LRYKGYSKALNGFVK	succinylation	[5]
732	LRYKGYSKALNGFVK	ubiquitination	[6]
739	KALNGFVKLGLINRE	acetylation	[1]
739	KALNGFVKLGLINRE	ubiquitination	[6]
761	EANPLTWKQLLCDLV	acetylation	[1, 4, 5]
761	EANPLTWKQLLCDLV	succinylation	[5]
778	SRSSPCEKLKEVVFT	acetylation	[1, 2, 3, 5]
778	SRSSPCEKLKEVVFT	succinylation	[5]
778	SRSSPCEKLKEVVFT	ubiquitination	[6]
780	SSPCEKLKEVVFTKL	acetylation	[1, 2, 3, 4, 5]
780	SSPCEKLKEVVFTKL	succinylation	[5]
820	SIVDAFSKHLVSKLS	acetylation	[4]
825	FSKHLVSKLSYGPEE	ubiquitination	[6]
833	LSYGPEEKDMIVMRD	ubiquitination	[6]
901	GLMGPFTKEIYGPIL	acetylation	[2]
901	GLMGPFTKEIYGPIL	ubiquitination	[6]

[1] Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways.
Rardin MJ, Newman JC, Held JM, Cusack MP, Sorensen DJ, Li B, Schilling B, Mooney SD, Kahn CR, Verdin E, Gibson BW.
Proc Natl Acad Sci U S A. 2013 Apr 16;110(16):6601-6. [PMID: 23576753]
[2] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123]
[3] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
J Biol Chem. 2013 Jul 17;. [PMID: 23864654]
[4] Quantitative assessment of the impact of the gut microbiota on lysine epsilon-acetylation of host proteins using gnotobiotic mice.
Simon GM, Cheng J, Gordon JI.
Proc Natl Acad Sci U S A. 2012 Jul 10;109(28):11133-8. [PMID: 22733758]
[5] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
[6] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
[7] Mitochondrial acetylome analysis in a mouse model of alcohol-induced liver injury utilizing SIRT3 knockout mice.
Fritz KS, Galligan JJ, Hirschey MD, Verdin E, Petersen DR.
J Proteome Res. 2012 Mar 2;11(3):1633-43. [PMID: 22309199]
[8] Circadian acetylome reveals regulation of mitochondrial metabolic pathways.
Masri S, Patel VR, Eckel-Mahan KL, Peleg S, Forne I, Ladurner AG, Baldi P, Imhof A, Sassone-Corsi P.
Proc Natl Acad Sci U S A. 2013 Feb 26;110(9):3339-44. [PMID: 23341599]

Functional Description

Bifunctional enzyme that catalyzes the first two steps in lysine degradation. The N-terminal and the C-terminal contain lysine-oxoglutarate reductase and saccharopine dehydrogenase activity, respectively.

REGION 33 455 Lysine-ketoglutarate reductase.
REGION 477 926 Saccharopine dehydrogenase.

Complete proteome; Mitochondrion; Multifunctional enzyme; NAD; NADP; Oxidoreductase; Reference proteome; Transit peptide.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0005739; C:mitochondrion; TAS:UniProtKB.
GO:0047131; F:saccharopine dehydrogenase (NAD+, L-glutamate-forming) activity; IDA:UniProtKB.
GO:0047130; F:saccharopine dehydrogenase (NADP+, L-lysine-forming) activity; IEA:EC.
GO:0006091; P:generation of precursor metabolites and energy; TAS:UniProtKB.
GO:0019477; P:L-lysine catabolic process; IDA:MGI.
GO:0033512; P:L-lysine catabolic process to acetyl-CoA via saccharopine; IEA:UniProtKB-UniPathway.

IPR007886; AlaDH/PNT_N.
IPR007698; AlaDH/PNT_NAD(H)-bd.
IPR016040; NAD(P)-bd_dom.
IPR005097; Saccharopine_DH/HSpermid_syn.

PF01262; AlaDh_PNT_C
PF05222; AlaDh_PNT_N
PF03435; Saccharop_dh

SM01002; AlaDh_PNT_C
SM01003; AlaDh_PNT_N

PS00836; ALADH_PNT_1
PS00837; ALADH_PNT_2