CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-042849
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 Bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase 
Protein Synonyms/Alias
  
Gene Name
 GNE 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
183AGCPSYDKLLSAKNKubiquitination[1]
188YDKLLSAKNKDYMSIacetylation[1]
190KLLSAKNKDYMSIIRubiquitination[1, 2]
205MWLGDDVKSKDYIVAubiquitination[2, 3]
207LGDDVKSKDYIVALQubiquitination[2]
254PNIDAGSKEMVRVMRubiquitination[2]
263MVRVMRKKGIEHHPNubiquitination[2]
337RDADTQDKILQALHLubiquitination[2]
348ALHLQFGKQYPCSKIubiquitination[2]
354GKQYPCSKIYGDGNAubiquitination[1, 2, 4]
370PRILKFLKSIDLQEPubiquitination[1, 2]
380DLQEPLQKKFCFPPVubiquitination[2]
381LQEPLQKKFCFPPVKubiquitination[2]
435KYTQFNPKTYEERINubiquitination[1]
485GIVLHSTKLIQEWNSubiquitination[2]
632AAKLGNAKAQSILRTubiquitination[2]
Reference
 [1] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094] 
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 717 AA 
Protein Sequence
MPIGDCSVAA KPRKQLLCSL FQTTLGYRAR ASGWKPMVIC RGSHAFKDLI NTYRMIEQDD 60
FDINTRLHTI VRGEDEAAMV ESVGLALVKL PDVLNRLKPD IMIVHGDRFD ALALATSAAL 120
MNIRILHIEG GEVSGTIDDS IRHAITKLAH YHVCCTRSAE QHLISMCEDH DRILLAGCPS 180
YDKLLSAKNK DYMSIIRMWL GDDVKSKDYI VALQHPVTTD IKHSIKMFEL TLDALISFNK 240
RTLVLFPNID AGSKEMVRVM RKKGIEHHPN FRAVKHVPFD QFIQLVAHAG CMIGNSSCGV 300
REVGAFGTPV INLGTRQIGR ETGENVLHVR DADTQDKILQ ALHLQFGKQY PCSKIYGDGN 360
AVPRILKFLK SIDLQEPLQK KFCFPPVKEN ISQDIDHILE TLSALAVDLG GTNLRVAIVS 420
MKGEIVKKYT QFNPKTYEER INLILQMCVE AAAEAVKLNC RILGVGISTG GRVNPREGIV 480
LHSTKLIQEW NSVDLRTPLS DTLHLPVWVD NDGNCAALAE RKFGQGKGLE NFVTLITGTG 540
IGGGIIHQHE LIHGSSFCAA ELGHLVVSLD GPDCSCGSHG CIEAYASGMA LQREAKKLHD 600
EDLLLVEGMS VPKDEAVGAL HLIQAAKLGN AKAQSILRTA GTALGLGVVN ILHTMNPSLV 660
ILSGVLASHY IHIVKDVIRQ QALSSVQDVD VVVSDLVDPA LLGAASMVLD YTTRRIY 717 
Gene Ontology
 GO:0005524; F:ATP binding; IEA:InterPro.
 GO:0009384; F:N-acylmannosamine kinase activity; IEA:InterPro.
 GO:0008761; F:UDP-N-acetylglucosamine 2-epimerase activity; IEA:InterPro.
 GO:0009103; P:lipopolysaccharide biosynthetic process; IEA:InterPro.
 GO:0046380; P:N-acetylneuraminate biosynthetic process; IEA:InterPro.
 GO:0006047; P:UDP-N-acetylglucosamine metabolic process; IEA:InterPro. 
Interpro
 IPR001312; Hexokinase.
 IPR000600; ROK.
 IPR020004; UDP-GlcNAc_Epase.
 IPR003331; UDP_GlcNAc_Epimerase_2. 
Pfam
 PF02350; Epimerase_2
 PF00480; ROK 
SMART
  
PROSITE
  
PRINTS
 PR00475; HEXOKINASE.