UniProt Accession

 UBE2T_MOUSE; Q9CQ37 

Genbank Protein ID

 AK012565; AK021213; BC029213 

Genbank Nucleotide ID

 BAB28320.1; BAB32332.1; AAH29213.1 

Protein Name

 Ubiquitin-conjugating enzyme E2 T 

Protein Synonyms/Alias

 Ubiquitin carrier protein T; Ubiquitin-protein ligase T 

Gene Name

 Ube2t 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
91	RICLDILKLPPKGAW	ubiquitination	[1]

Reference

 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 

Functional Description

 Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Catalyzes monoubiquitination. Involved in mitomycin-C (MMC)-induced DNA repair: acts as a specific E2 ubiquitin-conjugating enzyme for the Fanconi anemia complex by associating with E3 ubiquitin-protein ligase FANCL and catalyzing monoubiquitination of FANCD2, a key step in the DNA damage pathway. Also mediates monoubiquitination of FANCL and FANCI. May contribute to ubiquitination and degradation of BRCA1. In vitro able to promote polyubiquitination using all 7 ubiquitin Lys residues, but may prefer 'Lys-11'-, 'Lys-27'-, 'Lys-48'- and 'Lys-63'-linked polyubiquitination (By similarity). 

Sequence Annotation

 ACT_SITE 86 86 Glycyl thioester intermediate (By
 CROSSLNK 91 91 Glycyl lysine isopeptide (Lys-Gly)
 CROSSLNK 181 181 Glycyl lysine isopeptide (Lys-Gly)  

Keyword

 ATP-binding; Complete proteome; DNA damage; DNA repair; Isopeptide bond; Ligase; Nucleotide-binding; Nucleus; Reference proteome; Ubl conjugation; Ubl conjugation pathway. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 204 AA 

Protein Sequence

Gene Ontology

 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0003682; F:chromatin binding; ISS:UniProtKB.
 GO:0004842; F:ubiquitin-protein ligase activity; ISS:UniProtKB.
 GO:0006281; P:DNA repair; ISS:UniProtKB.
 GO:0051865; P:protein autoubiquitination; ISS:UniProtKB.
 GO:0070979; P:protein K11-linked ubiquitination; ISS:UniProtKB.
 GO:0044314; P:protein K27-linked ubiquitination; ISS:UniProtKB.
 GO:0035519; P:protein K29-linked ubiquitination; ISS:UniProtKB.
 GO:0070936; P:protein K48-linked ubiquitination; ISS:UniProtKB.
 GO:0085020; P:protein K6-linked ubiquitination; ISS:UniProtKB.
 GO:0070534; P:protein K63-linked ubiquitination; ISS:UniProtKB.
 GO:0006513; P:protein monoubiquitination; ISS:UniProtKB. 

Interpro

 IPR000608; UBQ-conjugat_E2.
 IPR023313; UBQ-conjugating_AS.
 IPR016135; UBQ-conjugating_enzyme/RWD. 

Pfam

 PF00179; UQ_con 

SMART

  

PROSITE

 PS00183; UBIQUITIN_CONJUGAT_1
 PS50127; UBIQUITIN_CONJUGAT_2 

PRINTS