UniProt Accession

 RBX1_YEAST; Q08273; D6W1T5 

Genbank Protein ID

 X95465; Z74876; BK006948 

Genbank Nucleotide ID

 CAA64737.1; CAA99155.1; DAA10651.1 

Protein Name

 RING-box protein HRT1 

Protein Synonyms/Alias

 RING-box protein 1; E3 ubiquitin-protein ligase complex SCF subunit HRT1; High level expression reduces Ty3 transposition protein 1 

Gene Name

 HRT1 

Gene Synonyms/Alias

 RBX1; YOL133W 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
31	QSAPVETKKKRFEIK	ubiquitination	[1]

Reference

 [1] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
 Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
 Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047] 

Functional Description

 Component of E3 ubiquitin ligase SCF complexes, which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. Seems to recruit the E2 ubiquitination enzyme, like CDC34/UBC3, to the complex and brings it into close proximity to the substrate. Also stimulates CDC34/UBC3 autoubiquitination and promotes the neddylation of CDC53. 

Sequence Annotation

 ZN_FING 55 111 RING-type.
 METAL 55 55 Zinc 1 (By similarity).
 METAL 58 58 Zinc 1 (By similarity).
 METAL 66 66 Zinc 3 (By similarity).
 METAL 69 69 Zinc 3 (By similarity).
 METAL 81 81 Zinc 3 (By similarity).
 METAL 88 88 Zinc 2 (By similarity).
 METAL 90 90 Zinc 2 (By similarity).
 METAL 93 93 Zinc 1 (By similarity).
 METAL 95 95 Zinc 3 (By similarity).
 METAL 96 96 Zinc 1 (By similarity).
 METAL 107 107 Zinc 2 (By similarity).
 METAL 110 110 Zinc 2 (By similarity).
 MOD_RES 15 15 Phosphoserine.  

Keyword

 Complete proteome; Cytoplasm; Direct protein sequencing; Metal-binding; Nucleus; Phosphoprotein; Reference proteome; Ubl conjugation pathway; Zinc; Zinc-finger. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 121 AA 

Protein Sequence

Gene Ontology

 GO:0031463; C:Cul3-RING ubiquitin ligase complex; IDA:SGD.
 GO:0035361; C:Cul8-RING ubiquitin ligase complex; IDA:SGD.
 GO:0005737; C:cytoplasm; IDA:SGD.
 GO:0005634; C:nucleus; IDA:SGD.
 GO:0019005; C:SCF ubiquitin ligase complex; IDA:SGD.
 GO:0030674; F:protein binding, bridging; IDA:SGD.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0000082; P:G1/S transition of mitotic cell cycle; IMP:SGD.
 GO:0042787; P:protein ubiquitination involved in ubiquitin-dependent protein catabolic process; IDA:SGD.
 GO:0031146; P:SCF-dependent proteasomal ubiquitin-dependent protein catabolic process; IDA:SGD. 

Interpro

 IPR001841; Znf_RING.
 IPR013083; Znf_RING/FYVE/PHD.
 IPR024766; Znf_RING_H2. 

Pfam

 PF12678; zf-rbx1 

SMART

 SM00184; RING 

PROSITE

 PS50089; ZF_RING_2 

PRINTS