CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-004229
UniProt Accession
Genbank Protein ID
 J04473 
Genbank Nucleotide ID
Protein Name
 Fumarate hydratase, mitochondrial 
Protein Synonyms/Alias
 Fumarase 
Gene Name
 Fh 
Gene Synonyms/Alias
 Fh1 
Created Date
 July 27, 2013 
Organism
 Rattus norvegicus (Rat) 
NCBI Taxa ID
 10116 
Lysine Modification
Position
Peptide
Type
References
58YDTFGELKVPTDKYYacetylation[1]
63ELKVPTDKYYGAQTVacetylation[1]
77VRSTMNFKIGGATERacetylation[1]
91RMPIPVIKAFGILKRacetylation[1]
97IKAFGILKRAAAEVNacetylation[1]
112QEYGLDPKIASAIMKacetylation[1]
119KIASAIMKAADEVAEacetylation[1]
128ADEVAEGKLNDHFPLacetylation[1]
169LGGELGSKKPVHPNDacetylation[1]
170GGELGSKKPVHPNDHacetylation[1]
220DALSAKSKEFAQVIKacetylation[1]
289TRIGFAEKVAAKVAAacetylation[1]
444ANTERINKLMNESLMacetylation[1]
464NPHIGYDKAAKIAKTacetylation[1]
467IGYDKAAKIAKTAHKacetylation[1]
470DKAAKIAKTAHKNGSacetylation[1]
499EQFDEWVKPKDMLGPacetylation[1]
Reference
 [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405] 
Functional Description
  
Sequence Annotation
 REGION 173 176 B site (By similarity).
 REGION 183 185 Substrate binding (By similarity).
 BINDING 144 144 Substrate (By similarity).
 MOD_RES 63 63 N6-acetyllysine (By similarity).
 MOD_RES 77 77 N6-acetyllysine (By similarity).
 MOD_RES 91 91 N6-acetyllysine (By similarity).
 MOD_RES 112 112 N6-acetyllysine (By similarity).
 MOD_RES 289 289 N6-acetyllysine (By similarity).
 MOD_RES 474 474 N6-acetyllysine (By similarity).  
Keyword
 Acetylation; Alternative initiation; Complete proteome; Cytoplasm; Direct protein sequencing; Lyase; Mitochondrion; Reference proteome; Transit peptide; Tricarboxylic acid cycle. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 507 AA 
Protein Sequence
MNRAFCLLAR SRRFPRVPSA GAVLSGEAAT LPRCAPNVVR MASQNSFRIE YDTFGELKVP 60
TDKYYGAQTV RSTMNFKIGG ATERMPIPVI KAFGILKRAA AEVNQEYGLD PKIASAIMKA 120
ADEVAEGKLN DHFPLVVWQT GSGTQTNMNV NEVISNRAIE MLGGELGSKK PVHPNDHVNK 180
SQSSNDTFPT AMHIAAALEV HQVLLPGLQK LHDALSAKSK EFAQVIKIGR THTQDAVPLT 240
LGQEFSGYVQ QVQYAMERIK AAMPRIYELA AGGTAVGTGL NTRIGFAEKV AAKVAALTGL 300
PFVTAPNKFE ALAAHDALVE LSGAMNTTAC SLMKIANDIR FLGSGPRSGL GELILPENEP 360
GSSIMPGKVN PTQCEAMTMV AAQVMGNHVA VTVGGSNGHF ELNVFKPMMI KNVLHSARLL 420
GDASVSFTEN CVVGIQANTE RINKLMNESL MLVTALNPHI GYDKAAKIAK TAHKNGSTLK 480
KTAIELGYLT AEQFDEWVKP KDMLGPK 507 
Gene Ontology
 GO:0005739; C:mitochondrion; IDA:RGD.
 GO:0045239; C:tricarboxylic acid cycle enzyme complex; IEA:InterPro.
 GO:0004333; F:fumarate hydratase activity; IDA:RGD.
 GO:0006106; P:fumarate metabolic process; IDA:RGD.
 GO:0006108; P:malate metabolic process; IDA:RGD.
 GO:0006099; P:tricarboxylic acid cycle; IDA:RGD. 
Interpro
 IPR005677; Fum_hydII.
 IPR024083; Fumarase/histidase_N.
 IPR018951; Fumarase_C_C.
 IPR000362; Fumarate_lyase.
 IPR020557; Fumarate_lyase_CS.
 IPR022761; Fumarate_lyase_N.
 IPR008948; L-Aspartase-like. 
Pfam
 PF10415; FumaraseC_C
 PF00206; Lyase_1 
SMART
  
PROSITE
 PS00163; FUMARATE_LYASES 
PRINTS
 PR00149; FUMRATELYASE.