UniProt Accession

 NHRF3_RAT; Q9JJ40; O35234; Q6AZ21 

Genbank Protein ID

 AF013145; AF116896; BC078788 

Genbank Nucleotide ID

 AAB66880.1; AAF74985.1; AAH78788.1 

Protein Name

 Na(+)/H(+) exchange regulatory cofactor NHE-RF3 

Protein Synonyms/Alias

 NHERF-3; C-terminal-linking and modulating protein; Dietary Pi-regulated RNA-1; Diphor-1; Na(+)/H(+) exchanger regulatory factor 3; Na/Pi cotransporter C-terminal-associated protein 1; NaPi-Cap1; PDZ domain-containing protein 1; Sodium-hydrogen exchanger regulatory factor 3 

Gene Name

 Pdzk1 

Gene Synonyms/Alias

 Clamp; Diphor1; Nherf3 

Created Date

 July 27, 2013 

Organism

 Rattus norvegicus (Rat) 

NCBI Taxa ID

 10116 

Lysine Modification

Position	Peptide	Type	References
28	GFFLRIEKDTDGHLV	acetylation	[1]
46	EEGSPAEKAGLLDGD	acetylation	[1]
65	INGVFVDKEEHAQVV	acetylation	[1]
97	DSYEKAVKHQVDLKE	acetylation	[1]
103	VKHQVDLKELDQSPR	acetylation	[1]
235	KRETASLKLLPHQPR	acetylation	[1]
269	EQKGQIIKDIEPGSP	acetylation	[1]
322	TTLLVLDKEADRIYS	acetylation	[1]
375	TEDVGDHKPKLCRLI	acetylation	[1]
383	PKLCRLIKEDDSYGF	acetylation	[1]

Reference

 [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405] 

Functional Description

 A scaffold protein that connects plasma membrane proteins and regulatory components, regulating their surface expression in epithelial cells apical domains. May be involved in the coordination of a diverse range of regulatory processes for ion transport and second messenger cascades. In complex with SLC9A3R1, may cluster proteins that are functionally dependent in a mutual fashion and modulate the trafficking and the activity of the associated membrane proteins. May play a role in the cellular mechanisms associated with multidrug resistance through its interaction with ABCC2 and PDZK1IP1. May potentiate the CFTR chloride channel activity (By similarity). May function to connect SCARB1 with the cellular machineries for intracellular cholesterol transport and/or metabolism. May be involved in the regulation of proximal tubular Na(+)-dependent inorganic phosphate cotransport therefore playing an important role in tubule function (By similarity). Component of a complex, composed of PDZK1, SYNGAP1, KLHL17 and NMDA receptors. Interacts (via PDZ1 domain) directly with KLHL17; the interaction is important for integrity of actin cytoskeleton structures in neurons. 

Sequence Annotation

 DOMAIN 9 90 PDZ 1.
 DOMAIN 128 215 PDZ 2.
 DOMAIN 243 323 PDZ 3.
 DOMAIN 378 458 PDZ 4.
 MOD_RES 492 492 Phosphoserine (By similarity).
 MOD_RES 495 495 Phosphoserine (By similarity).
 MOD_RES 518 518 Phosphoserine (By similarity).  

Keyword

 Complete proteome; Cytoplasm; Membrane; Phosphoprotein; Reference proteome; Repeat. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 523 AA 

Protein Sequence

Gene Ontology

 GO:0016324; C:apical plasma membrane; IDA:RGD.
 GO:0031526; C:brush border membrane; IEA:Compara.
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0045121; C:membrane raft; IDA:RGD.
 GO:0031528; C:microvillus membrane; IDA:RGD.
 GO:0005124; F:scavenger receptor binding; IDA:RGD.
 GO:0015879; P:carnitine transport; IEA:Compara.
 GO:0030301; P:cholesterol transport; NAS:RGD.
 GO:0090002; P:establishment of protein localization to plasma membrane; IEA:Compara.
 GO:0046907; P:intracellular transport; NAS:RGD.
 GO:0034767; P:positive regulation of ion transmembrane transport; IEA:Compara. 

Interpro

 IPR001478; PDZ. 

Pfam

 PF00595; PDZ 

SMART

 SM00228; PDZ 

PROSITE

 PS50106; PDZ 

PRINTS