UniProt Accession

 CH60_RAT; P63039; P19226; P19227; P97602 

Genbank Protein ID

 X54793; U68562; BC086507; X53585 

Genbank Nucleotide ID

 CAA38564.1; AAC53362.1; AAH86507.1; CAA37654.1 

Protein Name

 60 kDa heat shock protein, mitochondrial 

Protein Synonyms/Alias

 60 kDa chaperonin; Chaperonin 60; CPN60; HSP-65; Heat shock protein 60; HSP-60; Hsp60; Mitochondrial matrix protein P1 

Gene Name

 Hspd1 

Gene Synonyms/Alias

 Hsp60 

Created Date

 July 27, 2013 

Organism

 Rattus norvegicus (Rat) 

NCBI Taxa ID

 10116 

Lysine Modification

Position	Peptide	Type	References
31	RAYAKDVKFGADARA	acetylation	[1]
91	IDLKDKYKNIGAKLV	acetylation	[1]
96	KYKNIGAKLVQDVAN	acetylation	[1]
130	IAKEGFEKISKGANP	acetylation	[1]
133	EGFEKISKGANPVEI	acetylation	[1]
156	DAVIAELKKQSKPVT	acetylation	[1]
160	AELKKQSKPVTTPEE	acetylation	[1]
180	TISANGDKDIGNIIS	acetylation	[1]
202	RKGVITVKDGKTLND	acetylation	[1]
205	VITVKDGKTLNDELE	acetylation	[1]
218	LEIIEGMKFDRGYIS	acetylation	[1]
269	EIANAHRKPLVIIAE	acetylation	[1]
314	DNRKNQLKDMAIATG	acetylation	[1]
369	GDKAHIEKRIQEITE	acetylation	[1]
387	ITTSEYEKEKLNERL	acetylation	[1]
396	KLNERLAKLSDGVAV	acetylation	[1]
455	IPALDSLKPANEDQK	acetylation	[1]
516	DFVNMVEKGIIDPTK	acetylation	[1]

Reference

 [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405] 

Functional Description

 Implicated in mitochondrial protein import and macromolecular assembly. May facilitate the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix. 

Sequence Annotation

 MOD_RES 70 70 Phosphoserine (By similarity).
 MOD_RES 82 82 N6-acetyllysine (By similarity).
 MOD_RES 125 125 N6-acetyllysine (By similarity).
 MOD_RES 130 130 N6-acetyllysine (By similarity).
 MOD_RES 133 133 N6-malonyllysine (By similarity).
 MOD_RES 202 202 N6-acetyllysine (By similarity).
 MOD_RES 218 218 N6-acetyllysine (By similarity).
 MOD_RES 223 223 Phosphotyrosine (By similarity).
 MOD_RES 269 269 N6-acetyllysine (By similarity).
 MOD_RES 352 352 N6-acetyllysine (By similarity).
 MOD_RES 359 359 N6-acetyllysine (By similarity).
 MOD_RES 396 396 N6-acetyllysine (By similarity).
 MOD_RES 469 469 N6-acetyllysine (By similarity).  

Keyword

 Acetylation; ATP-binding; Chaperone; Complete proteome; Direct protein sequencing; Mitochondrion; Nucleotide-binding; Phosphoprotein; Reference proteome; Transit peptide. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 573 AA 

Protein Sequence

Gene Ontology

 GO:0009986; C:cell surface; IDA:RGD.
 GO:0005829; C:cytosol; IDA:RGD.
 GO:0005794; C:Golgi apparatus; IDA:RGD.
 GO:0045121; C:membrane raft; IDA:RGD.
 GO:0030061; C:mitochondrial crista; IDA:RGD.
 GO:0005759; C:mitochondrial matrix; IEA:UniProtKB-SubCell.
 GO:0005791; C:rough endoplasmic reticulum; IDA:RGD.
 GO:0042588; C:zymogen granule; IDA:RGD.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0043559; F:insulin binding; IPI:RGD.
 GO:0051085; P:chaperone mediated protein folding requiring cofactor; IDA:RGD.
 GO:0002236; P:detection of misfolded protein; IDA:RGD.
 GO:0043524; P:negative regulation of neuron apoptotic process; IMP:RGD.
 GO:0050729; P:positive regulation of inflammatory response; IDA:RGD.
 GO:0042026; P:protein refolding; IEA:InterPro.
 GO:0033198; P:response to ATP; IEP:RGD.
 GO:0042220; P:response to cocaine; IEP:RGD.
 GO:0042493; P:response to drug; IEP:RGD.
 GO:0043627; P:response to estrogen stimulus; IEP:RGD.
 GO:0009408; P:response to heat; IEP:RGD.
 GO:0042542; P:response to hydrogen peroxide; IEP:RGD.
 GO:0001666; P:response to hypoxia; IEP:RGD.
 GO:0032496; P:response to lipopolysaccharide; IEP:RGD. 

Interpro

 IPR018370; Chaperonin_Cpn60_CS.
 IPR001844; Chaprnin_Cpn60.
 IPR002423; Cpn60/TCP-1.
 IPR027409; GroEL-like_apical_dom.
 IPR027413; GROEL-like_equatorial. 

Pfam

 PF00118; Cpn60_TCP1 

SMART

  

PROSITE

 PS00296; CHAPERONINS_CPN60 

PRINTS

 PR00298; CHAPERONIN60.