CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-012291
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Translation initiation factor eIF-2B subunit alpha 
Protein Synonyms/Alias
 eIF-2B GDP-GTP exchange factor subunit alpha 
Gene Name
 EIF2B1 
Gene Synonyms/Alias
 EIF2BA 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
11KELIEYFKSQMKEDPubiquitination[1]
15EYFKSQMKEDPDMASubiquitination[1]
35RTLLEFLKRDKGETIacetylation[2]
35RTLLEFLKRDKGETIubiquitination[3, 4]
38LEFLKRDKGETIQGLubiquitination[5]
162SQPDLSGKKMAKALCubiquitination[1]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [5] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 
Functional Description
 Catalyzes the exchange of eukaryotic initiation factor 2-bound GDP for GTP. 
Sequence Annotation
 MOD_RES 35 35 N6-acetyllysine.  
Keyword
 3D-structure; Acetylation; Complete proteome; Disease mutation; Initiation factor; Leukodystrophy; Protein biosynthesis; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 305 AA 
Protein Sequence
MDDKELIEYF KSQMKEDPDM ASAVAAIRTL LEFLKRDKGE TIQGLRANLT SAIETLCGVD 60
SSVAVSSGGE LFLRFISLAS LEYSDYSKCK KIMIERGELF LRRISLSRNK IADLCHTFIK 120
DGATILTHAY SRVVLRVLEA AVAAKKRFSV YVTESQPDLS GKKMAKALCH LNVPVTVVLD 180
AAVGYIMEKA DLVIVGAEGV VENGGIINKI GTNQMAVCAK AQNKPFYVVA ESFKFVRLFP 240
LNQQDVPDKF KYKADTLKVA QTGQDLKEEH PWVDYTAPSL ITLLFTDLGV LTPSAVSDEL 300
IKLYL 305 
Gene Ontology
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005851; C:eukaryotic translation initiation factor 2B complex; IDA:UniProtKB.
 GO:0005886; C:plasma membrane; IDA:MGI.
 GO:0030234; F:enzyme regulator activity; IEA:Compara.
 GO:0019003; F:GDP binding; IEA:Compara.
 GO:0005525; F:GTP binding; IEA:Compara.
 GO:0003743; F:translation initiation factor activity; IEA:UniProtKB-KW.
 GO:0051716; P:cellular response to stimulus; IDA:UniProtKB.
 GO:0032057; P:negative regulation of translational initiation in response to stress; IEA:Compara.
 GO:0014003; P:oligodendrocyte development; IMP:UniProtKB.
 GO:0006446; P:regulation of translational initiation; IBA:RefGenome.
 GO:0009749; P:response to glucose stimulus; ISS:UniProtKB.
 GO:0009408; P:response to heat; ISS:UniProtKB.
 GO:0043434; P:response to peptide hormone stimulus; ISS:UniProtKB.
 GO:0006413; P:translational initiation; IDA:UniProtKB. 
Interpro
 IPR000649; IF-2B-related. 
Pfam
 PF01008; IF-2B 
SMART
  
PROSITE
  
PRINTS