CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-020021
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Condensin complex subunit 3 
Protein Synonyms/Alias
 Chromosome-associated protein G; Condensin subunit CAP-G; hCAP-G; Melanoma antigen NY-MEL-3; Non-SMC condensin I complex subunit G; XCAP-G homolog 
Gene Name
 NCAPG 
Gene Synonyms/Alias
 CAPG; NYMEL3 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
11ERRLLSIKEAFRLAQubiquitination[1]
26QPHQNQAKLVVALSRubiquitination[1]
142IDDDVFDKINKAMLIubiquitination[1, 2, 3]
145DVFDKINKAMLIRLKubiquitination[1, 2]
152KAMLIRLKDKIPNVRubiquitination[1]
208SCIAPSAKTLPKIVGubiquitination[1, 4]
212PSAKTLPKIVGRTKDubiquitination[1]
226DVKEAVRKLAYQVLAubiquitination[1]
235AYQVLAEKVHMRAMSubiquitination[1]
262NDRSDAVKQAMQKHLubiquitination[2]
496ELKMAEIKVKLIEAKubiquitination[1, 2]
498KMAEIKVKLIEAKEAubiquitination[1]
522FNRASELKEEIKALEubiquitination[1, 2, 3]
537DARINLLKETEQLEIubiquitination[1, 2]
690VEETATAKNVLKLLSubiquitination[2]
852PEIRVYTKALSSLELubiquitination[1, 5, 6]
899IQLEKGNKEFGDQAEubiquitination[2]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [4] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [5] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [6] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 Regulatory subunit of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases. 
Sequence Annotation
 REPEAT 94 131 HEAT 1.
 REPEAT 138 173 HEAT 2.
 REPEAT 174 212 HEAT 3.
 REPEAT 238 275 HEAT 4.
 REPEAT 276 313 HEAT 5.
 REPEAT 399 436 HEAT 6.
 REPEAT 439 478 HEAT 7.
 REPEAT 617 654 HEAT 8.
 REPEAT 687 724 HEAT 9.
 REPEAT 865 907 HEAT 10.
 MOD_RES 390 390 Phosphoserine.
 MOD_RES 674 674 Phosphoserine.
 MOD_RES 931 931 Phosphothreonine.
 MOD_RES 973 973 Phosphoserine.
 MOD_RES 975 975 Phosphoserine.
 MOD_RES 1002 1002 Phosphoserine.
 MOD_RES 1015 1015 Phosphoserine.  
Keyword
 Cell cycle; Cell division; Chromosome; Complete proteome; Cytoplasm; DNA condensation; Mitosis; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1015 AA 
Protein Sequence
MGAERRLLSI KEAFRLAQQP HQNQAKLVVA LSRTYRTMDD KTVFHEEFIH YLKYVMVVYK 60
REPAVERVIE FAAKFVTSFH QSDMEDDEEE EDGGLLNYLF TFLLKSHEAN SNAVRFRVCL 120
LINKLLGSMP ENAQIDDDVF DKINKAMLIR LKDKIPNVRI QAVLALSRLQ DPKDDECPVV 180
NAYATLIEND SNPEVRRAVL SCIAPSAKTL PKIVGRTKDV KEAVRKLAYQ VLAEKVHMRA 240
MSIAQRVMLL QQGLNDRSDA VKQAMQKHLL QGWLRFSEGN ILELLHRLDV ENSSEVAVSV 300
LNALFSITPL SELVGLCKNN DGRKLIPVET LTPEIALYWC ALCEYLKSKG DEGEEFLEQI 360
LPEPVVYADY LLSYIQSIPV VNEEHRGDFS YIGNLMTKEF IGQQLILIIK SLDTSEEGGR 420
KKLLAVLQEI LILPTIPISL VSFLVERLLH IIIDDNKRTQ IVTEIISEIR APIVTVGVNN 480
DPADVRKKEL KMAEIKVKLI EAKEALENCI TLQDFNRASE LKEEIKALED ARINLLKETE 540
QLEIKEVHIE KNDAETLQKC LILCYELLKQ MSISTGLSAT MNGIIESLIL PGIISIHPVV 600
RNLAVLCLGC CGLQNQDFAR KHFVLLLQVL QIDDVTIKIS ALKAIFDQLM TFGIEPFKTK 660
KIKTLHCEGT EINSDDEQES KEVEETATAK NVLKLLSDFL DSEVSELRTG AAEGLAKLMF 720
SGLLVSSRIL SRLILLWYNP VTEEDVQLRH CLGVFFPVFA YASRTNQECF EEAFLPTLQT 780
LANAPASSPL AEIDITNVAE LLVDLTRPSG LNPQAKTSQD YQALTVHDNL AMKICNEILT 840
SPCSPEIRVY TKALSSLELS SHLAKDLLVL LNEILEQVKD RTCLRALEKI KIQLEKGNKE 900
FGDQAEAAQD ATLTTTTFQN EDEKNKEVYM TPLRGVKATQ ASKSTQLKTN RGQRKVTVSA 960
RTNRRCQTAE ADSESDHEVP EPESEMKMRL PRRAKTAALE KSKLNLAQFL NEDLS 1015 
Gene Ontology
 GO:0015629; C:actin cytoskeleton; IDA:HPA.
 GO:0005813; C:centrosome; IDA:HPA.
 GO:0000796; C:condensin complex; IDA:UniProtKB.
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005634; C:nucleus; NAS:UniProtKB.
 GO:0051301; P:cell division; IEA:UniProtKB-KW.
 GO:0000278; P:mitotic cell cycle; TAS:Reactome.
 GO:0007076; P:mitotic chromosome condensation; IDA:UniProtKB. 
Interpro
 IPR011989; ARM-like.
 IPR016024; ARM-type_fold.
 IPR027165; CND3.
 IPR025977; Cnd3_C. 
Pfam
 PF12719; Cnd3 
SMART
  
PROSITE
 PS50077; HEAT_REPEAT 
PRINTS