UniProt Accession

 ATIF1_HUMAN; Q9UII2; Q5JXL8; Q6IAQ7; Q9BSL9 

Genbank Protein ID

 AB029042; AK316600; DB030607; AL050386; AF114836; AY005470; AL583540; BT009849; CR457097; AL353622; CH471059; BC004955; BC009677 

Genbank Nucleotide ID

 BAA88422.1; BAG38187.1; CAI46227.1; AAP97235.1; AAF97495.1; AAP88851.1; CAG33378.1; CAI19134.1; EAX07704.1; AAH04955.1; AAH09677.1 

Protein Name

 ATPase inhibitor, mitochondrial 

Protein Synonyms/Alias

 Inhibitor of F(1)F(o)-ATPase; IF(1); IF1 

Gene Name

 ATPIF1 

Gene Synonyms/Alias

 ATPI 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
82	EEEIVHHKKEIERLQ	acetylation	[1, 2]
90	KEIERLQKEIERHKQ	acetylation	[2]
90	KEIERLQKEIERHKQ	ubiquitination	[3]

Reference

 [1] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 

Functional Description

 Endogenous F(1)F(o)-ATPase inhibitor limiting ATP depletion when the mitochondrial membrane potential falls below a threshold and the F(1)F(o)-ATP synthase starts hydrolyzing ATP to pump protons out of the mitochondrial matrix. Required to avoid the consumption of cellular ATP when the F(1)F(o)-ATP synthase enzyme acts as an ATP hydrolase. Indirectly acts as a regulator of heme synthesis in erythroid tissues: regulates heme synthesis by modulating the mitochondrial pH and redox potential, allowing FECH to efficiently catalyze the incorporation of iron into protoporphyrin IX to produce heme. 

Sequence Annotation

 REGION 26 52 N-terminal inhibitory region (By
 REGION 74 106 Antiparallel alpha-helical coiled coil  

Keyword

 Alternative splicing; Coiled coil; Complete proteome; Direct protein sequencing; Mitochondrion; Reference proteome; Transit peptide. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 106 AA 

Protein Sequence

Gene Ontology

 GO:0009986; C:cell surface; IDA:UniProtKB.
 GO:0005739; C:mitochondrion; IDA:UniProtKB.
 GO:0043532; F:angiostatin binding; IDA:UniProtKB.
 GO:0051117; F:ATPase binding; IDA:UniProtKB.
 GO:0042030; F:ATPase inhibitor activity; IDA:UniProtKB.
 GO:0005516; F:calmodulin binding; ISS:UniProtKB.
 GO:0042803; F:protein homodimerization activity; ISS:UniProtKB.
 GO:0001525; P:angiogenesis; TAS:UniProtKB.
 GO:0030218; P:erythrocyte differentiation; IMP:UniProtKB.
 GO:0006091; P:generation of precursor metabolites and energy; TAS:ProtInc.
 GO:0006783; P:heme biosynthetic process; IMP:UniProtKB.
 GO:0001937; P:negative regulation of endothelial cell proliferation; IDA:UniProtKB.
 GO:0051346; P:negative regulation of hydrolase activity; IDA:UniProtKB.
 GO:0045980; P:negative regulation of nucleotide metabolic process; IEA:InterPro.
 GO:0051289; P:protein homotetramerization; ISS:UniProtKB.
 GO:0072593; P:reactive oxygen species metabolic process; IEA:Compara.
 GO:0042981; P:regulation of apoptotic process; IEA:Compara. 

Interpro

 IPR007648; ATPase_inhibitor_IATP_mt. 

Pfam

 PF04568; IATP 

SMART

  

PROSITE

  

PRINTS