CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-044381
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 Signal transducer and activator of transcription 1-alpha/beta 
Protein Synonyms/Alias
  
Gene Name
 STAT1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
195GVAKSDQKQEQLLLKubiquitination[1]
203QEQLLLKKMYLMLDNacetylation[2]
203QEQLLLKKMYLMLDNubiquitination[1]
242NDELVEWKRRQQSACubiquitination[3]
298YEHDPITKNKQVLWDubiquitination[3]
381NTVKGFRKFNILGTHubiquitination[1]
412EFRHLQLKEQKNAGTubiquitination[3]
513WQFSSVTKRGLNVDQubiquitination[3]
527QLNMLGEKLLGPNASubiquitination[3]
594ERERALLKDQQPGTFubiquitination[1, 3, 4, 5]
638HAVEPYTKKELSAVTubiquitination[5]
639AVEPYTKKELSAVTFubiquitination[1]
654PDIIRNYKVMAAENIubiquitination[1, 3, 5, 6, 7]
667NIPENPLKYLYPNIDubiquitination[1, 3, 5]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Proteomic investigations reveal a role for RNA processing factor THRAP3 in the DNA damage response.
 Beli P, Lukashchuk N, Wagner SA, Weinert BT, Olsen JV, Baskcomb L, Mann M, Jackson SP, Choudhary C.
 Mol Cell. 2012 Apr 27;46(2):212-25. [PMID: 22424773]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [5] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [6] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [7] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 714 AA 
Protein Sequence
MWMSQWYELQ QLDSKFLEQV HQLYDDSFPM EIRQYLAQWL EKQDWEHAAN DVSFATIRFH 60
DLLSQLDDQY SRFSLENNFL LQHNIRKSKR NLQDNFQEDP IQMSMIIYSC LKEERKILEN 120
AQRFNQAQSG NIQSTVMLDK QKELDSKVRN VKDKVMCIEH EIKSLEDLQD EYDFKCKTLQ 180
NREHETNGVA KSDQKQEQLL LKKMYLMLDN KRKEVVHKII ELLNVTELTQ NALINDELVE 240
WKRRQQSACI GGPPNACLDQ LQNWFTIVAE SLQQVRQQLK KLEELEQKYT YEHDPITKNK 300
QVLWDRTFSL FQQLIQSSFV VERQPCMPTH PQRPLVLKTG VQFTVKLRLL VKLQELNYNL 360
KVKVLFDKDV NERNTVKGFR KFNILGTHTK VMNMEESTNG SLAAEFRHLQ LKEQKNAGTR 420
TNEGPLIVTE ELHSLSFETQ LCQPGLVIDL ETTSLPVVVI SNVSQLPSGW ASILWYNMLV 480
AEPRNLSFFL TPPCARWAQL SEVLSWQFSS VTKRGLNVDQ LNMLGEKLLG PNASPDGLIP 540
WTRFCKENIN DKNFPFWLWI ESILELIKKH LLPLWNDGCI MGFISKERER ALLKDQQPGT 600
FLLRFSESSR EGAITFTWVE RSQNGGEPDF HAVEPYTKKE LSAVTFPDII RNYKVMAAEN 660
IPENPLKYLY PNIDKDHAFG KYYSRPKEAP EPMELDGPKG TGYIKTELIS VSEV 714 
Gene Ontology
 GO:0005634; C:nucleus; IEA:InterPro.
 GO:0005509; F:calcium ion binding; IEA:InterPro.
 GO:0003700; F:sequence-specific DNA binding transcription factor activity; IEA:InterPro.
 GO:0004871; F:signal transducer activity; IEA:InterPro. 
Interpro
 IPR011992; EF-hand-like_dom.
 IPR008967; p53-like_TF_DNA-bd.
 IPR000980; SH2.
 IPR013800; STAT_TF_alpha.
 IPR015988; STAT_TF_coiled-coil.
 IPR001217; STAT_TF_core.
 IPR013801; STAT_TF_DNA-bd.
 IPR012345; STAT_TF_DNA-bd_sub.
 IPR013799; STAT_TF_prot_interaction. 
Pfam
 PF00017; SH2
 PF01017; STAT_alpha
 PF02864; STAT_bind
 PF02865; STAT_int 
SMART
 SM00252; SH2
 SM00964; STAT_int 
PROSITE
 PS50001; SH2 
PRINTS