UniProt Accession

 1433F_HUMAN; Q04917 

Genbank Protein ID

 L20422; X80536; X78138; X57345; D78577; S80794; CR456612; Z82248; BC003047 

Genbank Nucleotide ID

 AAA35483.1; CAA56676.1; CAA55017.1; CAA40620.1; BAA11418.1; AAB36036.1; CAG30498.1; CAB05112.1; AAH03047.1 

Protein Name

 14-3-3 protein eta 

Protein Synonyms/Alias

 Protein AS1 

Gene Name

 YWHAH 

Gene Synonyms/Alias

 YWHA1 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
50	NLLSVAYKNVVGARR	ubiquitination	[1, 2, 3, 4]
69	VISSIEQKTMADGNE	ubiquitination	[1, 4]
77	TMADGNEKKLEKVKA	ubiquitination	[4, 5]
110	LLDKFLIKNCNDFQY	ubiquitination	[1, 3]
125	ESKVFYLKMKGDYYR	ubiquitination	[3]
127	KVFYLKMKGDYYRYL	ubiquitination	[3]
142	AEVASGEKKNSVVEA	ubiquitination	[4, 5]
143	EVASGEKKNSVVEAS	ubiquitination	[4]

Reference

 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [3] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [4] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [5] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 

Functional Description

 Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negatively regulates the kinase activity of PDPK1. 

Sequence Annotation

 MOD_RES 2 2 N-acetylglycine.
 MOD_RES 25 25 Phosphoserine.
 MOD_RES 59 59 Phosphoserine (By similarity).
 MOD_RES 117 117 Phosphotyrosine (By similarity).  

Keyword

 3D-structure; Acetylation; Complete proteome; Direct protein sequencing; Phosphoprotein; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 246 AA 

Protein Sequence

Gene Ontology

 GO:0030659; C:cytoplasmic vesicle membrane; TAS:Reactome.
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0014704; C:intercalated disc; IC:BHF-UCL.
 GO:0005159; F:insulin-like growth factor receptor binding; ISS:UniProtKB.
 GO:0044325; F:ion channel binding; ISS:BHF-UCL.
 GO:0019904; F:protein domain specific binding; ISS:UniProtKB.
 GO:0017080; F:sodium channel regulator activity; ISS:BHF-UCL.
 GO:0016044; P:cellular membrane organization; TAS:Reactome.
 GO:0006713; P:glucocorticoid catabolic process; IDA:UniProtKB.
 GO:0042921; P:glucocorticoid receptor signaling pathway; IDA:UniProtKB.
 GO:0006886; P:intracellular protein transport; ISS:UniProtKB.
 GO:0097193; P:intrinsic apoptotic signaling pathway; TAS:Reactome.
 GO:0050774; P:negative regulation of dendrite morphogenesis; ISS:UniProtKB.
 GO:1900740; P:positive regulation of protein insertion into mitochondrial membrane involved in apoptotic signaling pathway; TAS:Reactome.
 GO:0045893; P:positive regulation of transcription, DNA-dependent; IDA:UniProtKB.
 GO:2000649; P:regulation of sodium ion transmembrane transporter activity; ISS:BHF-UCL.
 GO:0048167; P:regulation of synaptic plasticity; ISS:UniProtKB. 

Interpro

 IPR000308; 14-3-3.
 IPR023409; 14-3-3_CS.
 IPR023410; 14-3-3_domain. 

Pfam

 PF00244; 14-3-3 

SMART

 SM00101; 14_3_3 

PROSITE

 PS00796; 1433_1
 PS00797; 1433_2 

PRINTS

 PR00305; 1433ZETA.