CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-012251
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Ubiquitin conjugation factor E4 A 
Protein Synonyms/Alias
  
Gene Name
 UBE4A 
Gene Synonyms/Alias
 KIAA0126 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
33KQFAAIQKEQLKQQSubiquitination[1]
104DNSDPSLKSGNGIPSubiquitination[1, 2, 3]
185YSCFQRAKEEITKVPubiquitination[1]
190RAKEEITKVPENLLPubiquitination[1]
324FVEYIQPKDPTNGQMubiquitination[1]
458KLCQPFCKPRSSRLLubiquitination[1]
475NPTYCALKELNDEERubiquitination[1]
485NDEERKIKNVHMRGLubiquitination[1]
494VHMRGLDKETCLIPAubiquitination[1]
538RLHDQMVKINQNLHRubiquitination[1]
773DTYRESIKDLADYASubiquitination[1, 4]
781DLADYASKNLEAMNPubiquitination[3, 4]
839TPEARREKEAGLQMFubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [3] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 Binds to the ubiquitin moieties of preformed conjugates and catalyzes ubiquitin chain assembly in conjunction with E1, E2, and E3 (By similarity). 
Sequence Annotation
 DOMAIN 991 1054 U-box.
 MOD_RES 386 386 N6-acetyllysine.  
Keyword
 3D-structure; Acetylation; Alternative splicing; Complete proteome; Direct protein sequencing; Reference proteome; Ubl conjugation pathway. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1066 AA 
Protein Sequence
MTDQENNNNI SSNPFAALFG SLADAKQFAA IQKEQLKQQS DELPASPDDS DNSVSESLDE 60
FDYSVAEISR SFRSQQEICE QLNINHMIQR IFLITLDNSD PSLKSGNGIP SRCVYLEEMA 120
VELEDQDWLD MSNVEQALFA RLLLQDPGNH LINMTSSTTL NLSADRDAGE RHIFCYLYSC 180
FQRAKEEITK VPENLLPFAV QCRNLTVSNT RTVLLTPEIY VDQNIHEQLV DLMLEAIQGA 240
REYMNKIYFE DVTEFLEEVI EALILDEEVR TFPEVMIPVF DILLGRIKDL ELCQILLYAY 300
LDILLYFTRQ KDMAKVFVEY IQPKDPTNGQ MYQKTLLGVI LSISCLLKTP GVVENHGYFL 360
NPSRSSPQEI KVQEANIHQF MAQFHEKIYQ MLKNLLQLSP ETKHCILSWL GNCLHANAGR 420
TKIWANQMPE IFFQMYASDA FFLNLGAALL KLCQPFCKPR SSRLLTFNPT YCALKELNDE 480
ERKIKNVHMR GLDKETCLIP AVQEPKFPQN YNLVTENLAL TEYTLYLGFH RLHDQMVKIN 540
QNLHRLQVAW RDAQQSSSPA ADNLREQFER LMTIYLSTKT AMTEPQMLQN CLNLQVSMAV 600
LLVQLAIGNE GSQPIELTFP LPDGYSSLAY VPEFFADNLG DFLIFLRRFA DDILETSADS 660
LEHVLHFITI FTGSIERMKN PHLRAKLAEV LEAVMPHLDQ TPNPLVSSVF HRKRVFCNFQ 720
YAPQLAEALI KVFVDIEFTG DPHQFEQKFN YRRPMYPILR YMWGTDTYRE SIKDLADYAS 780
KNLEAMNPPL FLRFLNLLMN DAIFLLDEAI QYLSKIKIQQ IEKDRGEWDS LTPEARREKE 840
AGLQMFGQLA RFHNIMSNET IGTLAFLTSE IKSLFVHPFL AERIISMLNY FLQHLVGPKM 900
GALKVKDFSE FDFKPQQLVS DICTIYLNLG DEENFCATVP KDGRSYSPTL FAQTVRVLKK 960
INKPGNMIMA FSNLAERIKS LADLQQQEEE TYADACDEFL DPIMSTLMCD PVVLPSSRVT 1020
VDRSTIARHL LSDQTDPFNR SPLTMDQIRP NTELKEKIQR WLAERKQQKE QLE 1073 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:Compara.
 GO:0000151; C:ubiquitin ligase complex; TAS:ProtInc.
 GO:0034450; F:ubiquitin-ubiquitin ligase activity; IEA:Compara.
 GO:0000209; P:protein polyubiquitination; IEA:Compara.
 GO:0006511; P:ubiquitin-dependent protein catabolic process; TAS:ProtInc. 
Interpro
 IPR019474; Ub_conjug_fac_E4_core.
 IPR003613; Ubox_domain.
 IPR013083; Znf_RING/FYVE/PHD. 
Pfam
 PF04564; U-box
 PF10408; Ufd2P_core 
SMART
 SM00504; Ubox 
PROSITE
  
PRINTS