| Tag | Content |
|---|
| CPLM ID | CPLM-011874 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | General negative regulator of transcription subunit 5 |
Protein Synonyms/Alias | |
Gene Name | NOT5 |
Gene Synonyms/Alias | YPR072W; YP9499.27 |
Created Date | July 27, 2013 |
Organism | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
NCBI Taxa ID | 559292 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 18 | DKLLKKVKEGIEDFD | ubiquitination | [1] | | 117 | LTNPDIIKDPKELKK | ubiquitination | [1] | | 170 | NLENILKKLQNNEMD | ubiquitination | [1] | | 298 | TVKDDSIKFDNSTLG | ubiquitination | [1] | | 314 | PTTHVSMKKKESEND | ubiquitination | [1] | | 338 | DRTDEIRKTIQHDVE | ubiquitination | [1, 2] | | 444 | NENDTDNKFGKDSKA | ubiquitination | [1] | | 526 | KLPPGMGKSEEESWR | ubiquitination | [1] |
|
Reference | [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation. Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J. Nat Methods. 2013 Jul;10(7):676-82. [ PMID: 23749301] [2] Sites of ubiquitin attachment in Saccharomyces cerevisiae. Starita LM, Lo RS, Eng JK, von Haller PD, Fields S. Proteomics. 2012 Jan;12(2):236-40. [ PMID: 22106047] |
Functional Description | Acts as component of the CCR4-NOT core complex, which in the nucleus seems to be a general transcription factor, and in the cytoplasm the major mRNA deadenylase involved in mRNA turnover. The NOT protein subcomplex negatively regulates the basal and activated transcription of many genes. Preferentially affects TC- type TATA element-dependent transcription. Could directly or indirectly inhibit component(s) of the general transcription machinery. |
Sequence Annotation | MOD_RES 306 306 Phosphothreonine.
MOD_RES 377 377 Phosphoserine. |
Keyword | Activator; Coiled coil; Complete proteome; Cytoplasm; Nucleus; Phosphoprotein; Reference proteome; Repressor; Transcription; Transcription regulation. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 560 AA |
Protein Sequence | MSQRKLQQDI DKLLKKVKEG IEDFDDIYEK FQSTDPSNSS HREKLESDLK REIKKLQKHR 60
DQIKTWLSKE DVKDKQSVLM TNRRLIENGM ERFKSVEKLM KTKQFSKEAL TNPDIIKDPK 120
ELKKRDQVLF IHDCLDELQK QLEQYEAQEN EEQTERHEFH IANLENILKK LQNNEMDPEP 180
VEEFQDDIKY YVENNDDPDF IEYDTIYEDM GCEIQPSSSN NEAPKEGNNQ TSLSSIRSSK 240
KQERSPKKKA PQRDVSISDR ATTPIAPGVE SASQSISSTP TPVSTDTPLH TVKDDSIKFD 300
NSTLGTPTTH VSMKKKESEN DSEQQLNFPP DRTDEIRKTI QHDVETNAAF QNPLFNDELK 360
YWLDSKRYLM QPLQEMSPKM VSQLESSLLN CPDSLDADSP CLYTKPLSLP HPTSIFFPNE 420
PIRFVYPYDV PLNLTNNEND TDNKFGKDSK AKSKKDDDIY SRTSLARIFM KFDLDTLFFI 480
FYHYQGSYEQ FLAARELFKN RNWLFNKVDR CWYYKEIEKL PPGMGKSEEE SWRYFDYKKS 540
WLARRCGNDF VYNEEDFEKL 560 |
Gene Ontology | GO:0030015; C:CCR4-NOT core complex; IPI:SGD. GO:0005737; C:cytoplasm; IDA:SGD. GO:0005634; C:nucleus; IEA:UniProtKB-SubCell. GO:0031087; P:deadenylation-independent decapping of nuclear-transcribed mRNA; IMP:SGD. GO:0000289; P:nuclear-transcribed mRNA poly(A) tail shortening; IDA:SGD. GO:0032968; P:positive regulation of transcription elongation from RNA polymerase II promoter; IDA:SGD. GO:0016567; P:protein ubiquitination; IMP:SGD. GO:0006368; P:transcription elongation from RNA polymerase II promoter; IMP:SGD. |
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PRINTS | |