UniProt Accession

 UB2J1_HUMAN; Q9Y385; A8K3F9; Q53F25; Q5W0N4; Q9BZ32; Q9NQL3; Q9NY66; Q9P011; Q9P0S0; Q9UF10 

Genbank Protein ID

 AJ245898; U93243; AF151834; AF151039; AF161502; AK290574; AK223464; AL138717; AL139804; AL139804; AL138717; CH471051; CH471051; BC013973 

Genbank Nucleotide ID

 CAB83212.1; AAF21505.1; AAD34071.1; AAF36125.1; AAF29117.1; BAF83263.1; BAD97184.1; CAH70228.1; CAH70228.1; CAI19635.1; CAI19635.1; EAW48555.1; EAW48556.1; AAH13973.1 

Protein Name

 Ubiquitin-conjugating enzyme E2 J1 

Protein Synonyms/Alias

 Non-canonical ubiquitin-conjugating enzyme 1; NCUBE-1; Yeast ubiquitin-conjugating enzyme UBC6 homolog E; HsUBC6e 

Gene Name

 UBE2J1 

Gene Synonyms/Alias

 NCUBE1; CGI-76; HSPC153; HSPC205 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
177	SQADQEAKELARQIS	ubiquitination	[1]
186	LARQISFKAEVNSSG	ubiquitination	[2, 3]

Reference

 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 

Functional Description

 Catalyzes the covalent attachment of ubiquitin to other proteins. Functions in the selective degradation of misfolded membrane proteins from the endoplasmic reticulum (ERAD). 

Sequence Annotation

 ACT_SITE 91 91 Glycyl thioester intermediate.
 MOD_RES 266 266 Phosphoserine.
 MOD_RES 268 268 Phosphoserine.  

Keyword

 ATP-binding; Complete proteome; Endoplasmic reticulum; Ligase; Membrane; Nucleotide-binding; Phosphoprotein; Polymorphism; Reference proteome; Transmembrane; Transmembrane helix; Ubl conjugation pathway. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 318 AA 

Protein Sequence

Gene Ontology

 GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004842; F:ubiquitin-protein ligase activity; IEA:EC.
 GO:0030433; P:ER-associated protein catabolic process; IMP:UniProtKB.
 GO:0018279; P:protein N-linked glycosylation via asparagine; IMP:UniProtKB. 

Interpro

 IPR000608; UBQ-conjugat_E2.
 IPR016135; UBQ-conjugating_enzyme/RWD. 

Pfam

 PF00179; UQ_con 

SMART

  

PROSITE

 PS00183; UBIQUITIN_CONJUGAT_1
 PS50127; UBIQUITIN_CONJUGAT_2 

PRINTS