UniProt Accession

 DCEA_ECOLI; P69908; P80063; Q2M7H9 

Genbank Protein ID

 M84024; X63123; U00039; U00096; AP009048 

Genbank Nucleotide ID

 AAA23833.1; CAA44834.1; AAB18493.1; AAC76542.1; BAE77777.1 

Protein Name

 Glutamate decarboxylase alpha 

Protein Synonyms/Alias

 GAD-alpha 

Gene Name

 gadA 

Gene Synonyms/Alias

 gadS; b3517; JW3485 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
4	****MDQKLLTDFRS	acetylation	[1, 2]
21	LDSRFGAKAISTIAE	acetylation	[2]
30	ISTIAESKRFPLHEM	acetylation	[1]
74	WDDENVHKLMDLSIN	acetylation	[1, 2]
87	INKNWIDKEEYPQSA	acetylation	[2]
150	KRMEAAGKPTDKPNL	acetylation	[1]
154	AAGKPTDKPNLVCGP	acetylation	[1]
192	GQLFMDPKRMIEACD	acetylation	[1, 2]
276	SISASGHKFGLAPLG	acetylation	[2]
341	LGREGYTKVQNASYQ	acetylation	[2]
381	GIPAVCFKLKDGEDP	acetylation	[1]
383	PAVCFKLKDGEDPGY	acetylation	[1, 2]
442	ELLLEDYKASLKYLS	acetylation	[2]
446	EDYKASLKYLSDHPK	acetylation	[1, 2, 3]
453	KYLSDHPKLQGIAQQ	acetylation	[1, 2, 3]
464	IAQQNSFKHT*****	acetylation	[1, 2, 3]

Reference

 [1] Comprehensive profiling of protein lysine acetylation in Escherichia coli.
 Zhang K, Zheng S, Yang JS, Chen Y, Cheng Z.
 J Proteome Res. 2013 Feb 1;12(2):844-51. [PMID: 23294111]
 [2] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618]
 [3] Lysine acetylation is a highly abundant and evolutionarily conserved modification in Escherichia coli.
 Zhang J, Sprung R, Pei J, Tan X, Kim S, Zhu H, Liu CF, Grishin NV, Zhao Y.
 Mol Cell Proteomics. 2009 Feb;8(2):215-25. [PMID: 18723842] 

Functional Description

 Converts glutamate to gamma-aminobutyrate (GABA), consuming one intracellular proton in the reaction. The gad system helps to maintain a near-neutral intracellular pH when cells are exposed to extremely acidic conditions. The ability to survive transit through the acidic conditions of the stomach is essential for successful colonization of the mammalian host by commensal and pathogenic bacteria. 

Sequence Annotation

 REGION 126 127 Pyridoxal phosphate binding (By
 BINDING 62 62 Substrate (By similarity).
 BINDING 83 83 Substrate (By similarity).
 BINDING 212 212 Pyridoxal phosphate (By similarity).
 BINDING 275 275 Pyridoxal phosphate (By similarity).
 MOD_RES 276 276 N6-(pyridoxal phosphate)lysine.  

Keyword

 3D-structure; Complete proteome; Decarboxylase; Direct protein sequencing; Lyase; Pyridoxal phosphate; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 466 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; IDA:UniProtKB.
 GO:0016020; C:membrane; IDA:UniProtKB.
 GO:0004351; F:glutamate decarboxylase activity; IDA:EcoCyc.
 GO:0030170; F:pyridoxal phosphate binding; IEA:InterPro.
 GO:0006536; P:glutamate metabolic process; IEA:InterPro.
 GO:0051454; P:intracellular pH elevation; IMP:EcoCyc. 

Interpro

 IPR010107; Glutamate_decarboxylase.
 IPR002129; PyrdxlP-dep_de-COase.
 IPR015424; PyrdxlP-dep_Trfase.
 IPR015421; PyrdxlP-dep_Trfase_major_sub1.
 IPR021115; Pyridoxal-P_BS. 

Pfam

 PF00282; Pyridoxal_deC 

SMART

  

PROSITE

 PS00392; DDC_GAD_HDC_YDC 

PRINTS