CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-037695
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 Nucleolar transcription factor 1 
Protein Synonyms/Alias
  
Gene Name
 UBTF 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
17DLEMAAPKGQDRWSQubiquitination[1]
45LPSNDSSKFKTTESHubiquitination[1, 2]
47SNDSSKFKTTESHMDubiquitination[1]
57ESHMDWEKVAFKDFSubiquitination[1]
61DWEKVAFKDFSGDMCubiquitination[1]
69DFSGDMCKLKWVEISubiquitination[1]
71SGDMCKLKWVEISNEubiquitination[1, 2, 3, 4]
97LDAQEHVKNPYKGKKubiquitination[1]
114KHPDFPKKPLTPYFRacetylation[2]
114KHPDFPKKPLTPYFRubiquitination[1, 2]
126YFRFFMEKRAKYAKLubiquitination[1]
132EKRAKYAKLHPEMSNubiquitination[1, 2, 3, 4, 5]
144MSNLDLTKILSKKYKubiquitination[1, 2]
151KILSKKYKELPEKKKubiquitination[1]
160LPEKKKMKYIQDFQRubiquitination[1, 3]
191PDLIQNAKKSDIPEKubiquitination[1]
212LWYTHEKKVYLKVRPubiquitination[1]
216HEKKVYLKVRPDEIMubiquitination[1, 3]
229IMRDYIQKHPELNISubiquitination[1, 2, 3, 5, 6]
242ISEEGITKSTLTKAEubiquitination[1, 2, 3, 6, 7]
247ITKSTLTKAERQLKDacetylation[2]
247ITKSTLTKAERQLKDubiquitination[1]
296VLCSQQWKLLSQKEKubiquitination[1, 4]
314HKKCDQKKKDYEVELubiquitination[1]
341QRVLGEEKMLNINKKubiquitination[1]
347EKMLNINKKQATSPAubiquitination[1]
348KMLNINKKQATSPASubiquitination[1]
415WNDLSEKKKAKYKARubiquitination[1]
443GEREERGKLPESPKRubiquitination[1, 2]
557ELNHLPLKERMVEIGubiquitination[1, 2]
590EEQQKQYKVHLDLWVubiquitination[1]
609PQDRAAYKEYISNKRubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [5] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [6] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [7] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 745 AA 
Protein Sequence
MNGEADCPTD LEMAAPKGQD RWSQEDMLTL LECMKNNLPS NDSSKFKTTE SHMDWEKVAF 60
KDFSGDMCKL KWVEISNEVR KFRTLTELIL DAQEHVKNPY KGKKLKKHPD FPKKPLTPYF 120
RFFMEKRAKY AKLHPEMSNL DLTKILSKKY KELPEKKKMK YIQDFQREKQ EFERNLARFR 180
EDHPDLIQNA KKSDIPEKPK TPQQLWYTHE KKVYLKVRPD EIMRDYIQKH PELNISEEGI 240
TKSTLTKAER QLKDKFDGRP TKPPPNSYSL YCAELMANMK DVPSTERMVL CSQQWKLLSQ 300
KEKDAYHKKC DQKKKDYEVE LLRFLESLPE EEQQRVLGEE KMLNINKKQA TSPASKKPAQ 360
EGGKGGSEKP KRPVSAMFIF SEEKRRQLQE ERPELSESEL TRLLARMWND LSEKKKAKYK 420
AREAALKAQS ERKPGGEREE RGKLPESPKR AEEIWQQSVI GDYLARFKND RVKALKAMEM 480
TWNNMEKKEK LMWIKKAAED QKRYERELSE MRAPPAATNS SKKMKFQGEP KKPPMNGYQK 540
FSQELLSNGE LNHLPLKERM VEIGSRWQRI SQSQKEHYKK LAEEQQKQYK VHLDLWVKSL 600
SPQDRAAYKE YISNKRKSMT KLRGPNPKSS RTTLQSKSPR RMMKRMRMTR TRMKKRKMMR 660
MGTPLKMAAT PLSPAARTRA RMGMRMKRMT RTKTTTRMTM RMKIMSPRAA APAPPPQGTP 720
QTLTPTEAQP HPRAARESPG APLPN 745 
Gene Ontology
 GO:0005730; C:nucleolus; IDA:HPA. 
Interpro
 IPR009071; HMG_box_dom. 
Pfam
 PF09011; DUF1898
 PF00505; HMG_box 
SMART
 SM00398; HMG 
PROSITE
 PS50118; HMG_BOX_2 
PRINTS