UniProt Accession

 SYP_SALTY; Q7CR62 

Genbank Protein ID

 AE006468 

Genbank Nucleotide ID

 AAL19205.1 

Protein Name

 Proline--tRNA ligase 

Protein Synonyms/Alias

 Prolyl-tRNA synthetase; ProRS 

Gene Name

 proS 

Gene Synonyms/Alias

 STM0242 

Created Date

 July 27, 2013 

Organism

 Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) 

NCBI Taxa ID

 99287 

Lysine Modification

Position	Peptide	Type	References
287	TLLVKAVKDSKSPLV	acetylation	[1]
290	VKAVKDSKSPLVALL	acetylation	[1]

Reference

 [1] Regulation of cellular metabolism by protein lysine acetylation.
 Zhao S, Xu W, Jiang W, Yu W, Lin Y, Zhang T, Yao J, Zhou L, Zeng Y, Li H, Li Y, Shi J, An W, Hancock SM, He F, Qin L, Chin J, Yang P, Chen X, Lei Q, Xiong Y, Guan KL.
 Science. 2010 Feb 19;327(5968):1000-4. [PMID: 20167786] 

Functional Description

 Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids such as alanine and cysteine, to avoid such errors it has two additional distinct editing activities against alanine. One activity is designated as 'pretransfer' editing and involves the tRNA(Pro)-independent hydrolysis of activated Ala-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Ala-tRNA(Pro). The misacylated Cys- tRNA(Pro) is not edited by ProRS (By similarity). 

Sequence Annotation

  

Keyword

 Aminoacyl-tRNA synthetase; ATP-binding; Complete proteome; Cytoplasm; Ligase; Nucleotide-binding; Protein biosynthesis; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 572 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0002161; F:aminoacyl-tRNA editing activity; IEA:InterPro.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004827; F:proline-tRNA ligase activity; IEA:EC.
 GO:0006433; P:prolyl-tRNA aminoacylation; IEA:InterPro.
 GO:0006450; P:regulation of translational fidelity; IEA:GOC. 

Interpro

 IPR002314; aa-tRNA-synt_IIb_cons-dom.
 IPR006195; aa-tRNA-synth_II.
 IPR004154; Anticodon-bd.
 IPR002316; Pro-tRNA-ligase_IIa.
 IPR004500; Pro-tRNA-synth_IIa_bac-type.
 IPR023717; Pro-tRNA-Synthase_IIa_type1.
 IPR007214; YbaK/aa-tRNA-synth-assoc-dom. 

Pfam

 PF03129; HGTP_anticodon
 PF00587; tRNA-synt_2b
 PF04073; YbaK 

SMART

  

PROSITE

 PS50862; AA_TRNA_LIGASE_II 

PRINTS

 PR01046; TRNASYNTHPRO.