UniProt Accession

 CAZA2_HUMAN; P47755 

Genbank Protein ID

 U03269; BT006735; AC002543; BC005338; U03851 

Genbank Nucleotide ID

 AAA88848.1; AAP35381.1; AAC60382.1; AAH05338.1; AAC00534.1 

Protein Name

 F-actin-capping protein subunit alpha-2 

Protein Synonyms/Alias

 CapZ alpha-2 

Gene Name

 CAPZA2 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
13	EQLSDEEKVRIAAKF	ubiquitination	[1]
19	EKVRIAAKFIIHAPP	ubiquitination	[1, 2, 3]
86	HGDLGNGKFLDPKNR	acetylation	[4]
86	HGDLGNGKFLDPKNR	ubiquitination	[3]
91	NGKFLDPKNRICFKF	ubiquitination	[1]
97	PKNRICFKFDHLRKE	acetylation	[4]
133	TALRAYVKEHYPNGV	ubiquitination	[2]
166	ESHQFQAKNFWNGRW	ubiquitination	[5]
226	SNEVQTAKEFIKIVE	ubiquitination	[2, 3]
256	TMSDTTFKALRRQLP	ubiquitination	[3, 5]
268	QLPVTRTKIDWNKIL	ubiquitination	[1, 6, 7]
273	RTKIDWNKILSYKIG	acetylation	[4, 8, 9, 10]
273	RTKIDWNKILSYKIG	ubiquitination	[1, 2, 3, 5, 6, 7, 10]
278	WNKILSYKIGKEMQN	acetylation	[10]
278	WNKILSYKIGKEMQN	ubiquitination	[1, 2, 3, 5, 10]
281	ILSYKIGKEMQNA**	ubiquitination	[1, 3, 6, 10]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [5] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [6] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [7] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [8] Proteomic investigations reveal a role for RNA processing factor THRAP3 in the DNA damage response.
 Beli P, Lukashchuk N, Wagner SA, Weinert BT, Olsen JV, Baskcomb L, Mann M, Jackson SP, Choudhary C.
 Mol Cell. 2012 Apr 27;46(2):212-25. [PMID: 22424773]
 [9] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [10] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 

Functional Description

 F-actin-capping proteins bind in a Ca(2+)-independent manner to the fast growing ends of actin filaments (barbed end) thereby blocking the exchange of subunits at these ends. Unlike other capping proteins (such as gelsolin and severin), these proteins do not sever actin filaments. 

Sequence Annotation

 MOD_RES 2 2 N-acetylalanine.  

Keyword

 Acetylation; Actin capping; Actin-binding; Complete proteome; Direct protein sequencing; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 286 AA 

Protein Sequence

Gene Ontology

 GO:0030863; C:cortical cytoskeleton; IEA:Compara.
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005576; C:extracellular region; TAS:Reactome.
 GO:0008290; C:F-actin capping protein complex; TAS:ProtInc.
 GO:0016020; C:membrane; IEA:Compara.
 GO:0030036; P:actin cytoskeleton organization; IEA:InterPro.
 GO:0051693; P:actin filament capping; IEA:UniProtKB-KW.
 GO:0007596; P:blood coagulation; TAS:Reactome.
 GO:0006928; P:cellular component movement; TAS:ProtInc.
 GO:0045087; P:innate immune response; TAS:Reactome.
 GO:0006461; P:protein complex assembly; TAS:ProtInc. 

Interpro

 IPR017865; F-actin_cap_asu_CS.
 IPR002189; WASH_F-actin_cap_alpha. 

Pfam

 PF01267; F-actin_cap_A 

SMART

  

PROSITE

 PS00748; F_ACTIN_CAPPING_A_1
 PS00749; F_ACTIN_CAPPING_A_2 

PRINTS

 PR00191; FACTINCAPA.