UniProt Accession

 SYYM_MOUSE; Q8BYL4; Q6PAH7 

Genbank Protein ID

 BC060295; AK039123 

Genbank Nucleotide ID

 AAH60295.1; BAC30245.1 

Protein Name

 Tyrosine--tRNA ligase, mitochondrial 

Protein Synonyms/Alias

 Tyrosyl-tRNA synthetase; TyrRS 

Gene Name

 Yars2 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
43	PRARGLFKEFFPESG	acetylation	[1]
362	QEGLDSAKRCTQALY	acetylation	[1, 2, 3]
362	QEGLDSAKRCTQALY	succinylation	[3]

Reference

 [1] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123]
 [2] Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways.
 Rardin MJ, Newman JC, Held JM, Cusack MP, Sorensen DJ, Li B, Schilling B, Mooney SD, Kahn CR, Verdin E, Gibson BW.
 Proc Natl Acad Sci U S A. 2013 Apr 16;110(16):6601-6. [PMID: 23576753]
 [3] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337] 

Functional Description

 Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr) (By similarity). 

Sequence Annotation

 MOTIF 77 86 "HIGH" region.
 MOTIF 276 280 "KMSKS" region.
 BINDING 279 279 ATP (By similarity).
 MOD_RES 350 350 N6-acetyllysine (By similarity).  

Keyword

 Acetylation; Aminoacyl-tRNA synthetase; ATP-binding; Complete proteome; Ligase; Mitochondrion; Nucleotide-binding; Protein biosynthesis; Reference proteome; Transit peptide. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 472 AA 

Protein Sequence

Gene Ontology

 GO:0005759; C:mitochondrial matrix; IEA:UniProtKB-SubCell.
 GO:0005739; C:mitochondrion; IDA:MGI.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0003723; F:RNA binding; IEA:InterPro.
 GO:0072545; F:tyrosine binding; IEA:Compara.
 GO:0004831; F:tyrosine-tRNA ligase activity; IEA:EC.
 GO:0006437; P:tyrosyl-tRNA aminoacylation; IEA:InterPro. 

Interpro

 IPR001412; aa-tRNA-synth_I_CS.
 IPR002305; aa-tRNA-synth_Ic.
 IPR014729; Rossmann-like_a/b/a_fold.
 IPR002942; S4_RNA-bd.
 IPR002307; Tyr-tRNA-ligase.
 IPR024088; Tyr-tRNA-ligase_bac-type. 

Pfam

 PF00579; tRNA-synt_1b 

SMART

  

PROSITE

 PS00178; AA_TRNA_LIGASE_I 

PRINTS

 PR01040; TRNASYNTHTYR.