UniProt Accession

 GDIB_HUMAN; P50395; O43928; Q5SX88; Q9UQM6 

Genbank Protein ID

 D13988; Y13286; Y13287; Y13288; Y13289; Y13290; Y13291; Y13292; Y13293; Y13294; Y13295; Y13296; Y13297; BT006868; AK297554; AL596094; BC005145; AF144713 

Genbank Nucleotide ID

 BAA03095.1; CAA73734.1; CAA73735.1; CAA73735.1; CAA73735.1; CAA73735.1; CAA73735.1; CAA73735.1; CAA73735.1; CAA73735.1; CAA73735.1; CAA73735.1; CAA73735.1; AAP35514.1; BAG59947.1; CAI13363.1; AAH05145.1; AAD34588.1 

Protein Name

 Rab GDP dissociation inhibitor beta 

Protein Synonyms/Alias

 Rab GDI beta; Guanosine diphosphate dissociation inhibitor 2; GDI-2 

Gene Name

 GDI2 

Gene Synonyms/Alias

 RABGDIB 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
54	TPLEDLYKRFKIPGS	ubiquitination	[1, 2]
112	TEGSFVYKGGKIYKV	acetylation	[3]
142	FEKRRFRKFLVYVAN	ubiquitination	[1, 4, 5]
174	TMRDVYKKFDLGQDV	ubiquitination	[5]
221	ESLARYGKSPYLYPL	ubiquitination	[1, 6]
253	GGTYMLNKPIEEIIV	ubiquitination	[2]
269	NGKVIGVKSEGEIAR	acetylation	[3]
293	YVKDRVEKVGQVIRV	ubiquitination	[5]
360	VSTTVETKEPEKEIR	ubiquitination	[2]
422	DDIKNIYKRMTGSEF	ubiquitination	[1]
435	EFDFEEMKRKKNDIY	ubiquitination	[2]

Reference

 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [3] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [4] Mass spectrometric analysis of lysine ubiquitylation reveals promiscuity at site level.
 Danielsen JM, Sylvestersen KB, Bekker-Jensen S, Szklarczyk D, Poulsen JW, Horn H, Jensen LJ, Mailand N, Nielsen ML.
 Mol Cell Proteomics. 2011 Mar;10(3):M110.003590. [PMID: 21139048]
 [5] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
 [6] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572] 

Functional Description

 Regulates the GDP/GTP exchange reaction of most Rab proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them. 

Sequence Annotation

 MOD_RES 1 1 N-acetylmethionine.
 MOD_RES 61 61 Phosphoserine.
 MOD_RES 112 112 N6-acetyllysine.
 MOD_RES 269 269 N6-acetyllysine.  

Keyword

 Acetylation; Alternative splicing; Complete proteome; Cytoplasm; GTPase activation; Membrane; Phosphoprotein; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 445 AA 

Protein Sequence

Gene Ontology

 GO:0009986; C:cell surface; IDA:UniProtKB.
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005794; C:Golgi apparatus; IEA:Compara.
 GO:0016020; C:membrane; IEA:UniProtKB-SubCell.
 GO:0005093; F:Rab GDP-dissociation inhibitor activity; TAS:ProtInc.
 GO:0005097; F:Rab GTPase activator activity; IEA:Compara.
 GO:0015031; P:protein transport; IEA:InterPro.
 GO:0051056; P:regulation of small GTPase mediated signal transduction; TAS:Reactome.
 GO:0007264; P:small GTPase mediated signal transduction; TAS:Reactome. 

Interpro

 IPR018203; GDP_dissociation_inhibitor.
 IPR000806; RabGDI. 

Pfam

 PF00996; GDI 

SMART

  

PROSITE

  

PRINTS

 PR00892; RABGDI.
 PR00891; RABGDIREP.