CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-022225
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Kelch-like protein 11 
Protein Synonyms/Alias
  
Gene Name
 KLHL11 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
142SGRVEMRKWSSEPGPubiquitination[1, 2, 3]
189RFLLIRLKEFCGEFLubiquitination[2]
234MIRRNFHKVIQDEEFubiquitination[1, 4]
290RYFEELFKLLRLSQMubiquitination[1, 2, 4]
298LLRLSQMKPTYLTRHubiquitination[1, 2, 5, 6]
307TYLTRHVKPERLVANubiquitination[1]
320ANNEVCVKLVADAVEubiquitination[2, 7]
443VCSLMTRKHSFGLTEubiquitination[2]
452SFGLTEVKGKLYSIGubiquitination[1, 2, 4, 8]
454GLTEVKGKLYSIGGHubiquitination[1, 2, 5, 6, 8]
469GNFSPGFKDVTVYNPubiquitination[1, 2, 3, 5, 6]
480VYNPELDKWHNLESAubiquitination[1, 2, 3, 5, 6, 8]
489HNLESAPKILRDVKAubiquitination[2, 5, 6]
495PKILRDVKALAIEDRubiquitination[1, 2, 3, 8]
521RDTEDGLKAVITCYDubiquitination[2, 4, 7, 8]
625KNSDDIDKQYRKEAYubiquitination[1, 2, 8]
682PQNLMWQKDRIRQMQubiquitination[1, 2, 3, 4, 5, 6, 9, 10]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [4] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [5] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [6] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [7] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
 [8] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [9] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [10] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965
Functional Description
 Component of a cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex that mediates the ubiquitination of target proteins, leading most often to their proteasomal degradation (By similarity). 
Sequence Annotation
 DOMAIN 94 170 BTB.
 DOMAIN 205 307 BACK.
 REPEAT 360 407 Kelch 1.
 REPEAT 408 453 Kelch 2.
 REPEAT 455 501 Kelch 3.
 REPEAT 503 556 Kelch 4.
 REPEAT 610 661 Kelch 5.  
Keyword
 3D-structure; Complete proteome; Kelch repeat; Reference proteome; Repeat; Signal; Ubl conjugation pathway. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 708 AA 
Protein Sequence
MAAAAVAAAA AAAAAASLQV LEMESMETAA AGSAGLAAEV RGSGTVDFGP GPGISAMEAS 60
GGDPGPEAED FECSSHCSEL SWRQNEQRRQ GLFCDITLCF GGAGGREFRA HRSVLAAATE 120
YFTPLLSGQF SESRSGRVEM RKWSSEPGPE PDTVEAVIEY MYTGRIRVST GSVHEVLELA 180
DRFLLIRLKE FCGEFLKKKL HLSNCVAIHS LAHMYTLSQL ALKAADMIRR NFHKVIQDEE 240
FYTLPFHLIR DWLSDLEITV DSEEVLFETV LKWVQRNAEE RERYFEELFK LLRLSQMKPT 300
YLTRHVKPER LVANNEVCVK LVADAVERHA LRAENIQSGT CQHPTSHVSL LPRYGQNMDV 360
IMVIGGVSEG GDYLSECVGY FVDEDRWVNL PHIHNHLDGH AVAVTESYVY VAGSMEPGFA 420
KTVERYNPNL NTWEHVCSLM TRKHSFGLTE VKGKLYSIGG HGNFSPGFKD VTVYNPELDK 480
WHNLESAPKI LRDVKALAIE DRFVYIAART PVDRDTEDGL KAVITCYDTE TRQWQDVESL 540
PLIDNYCFFQ MSVVNSNFYQ TASCCPKSYC LENEEAVRKI ASQVSDEILE SLPPEVLSIE 600
GAAICYYKDD VFIIGGWKNS DDIDKQYRKE AYRYCAERKR WMLLPPMPQP RCRATACHVR 660
IPYRYLHGTQ RYPMPQNLMW QKDRIRQMQE IHRHALNMRR VPSSQIEC 708 
Gene Ontology
  
Interpro
 IPR011705; BACK.
 IPR000210; BTB/POZ-like.
 IPR011333; BTB/POZ_fold.
 IPR013069; BTB_POZ.
 IPR015915; Kelch-typ_b-propeller.
 IPR006652; Kelch_1. 
Pfam
 PF07707; BACK
 PF00651; BTB
 PF01344; Kelch_1 
SMART
 SM00875; BACK
 SM00225; BTB
 SM00612; Kelch 
PROSITE
 PS50097; BTB 
PRINTS