UniProt Accession

 B7Z1C9_HUMAN; B7Z1C9 

Genbank Protein ID

 AC010913; AK293175; CH471053 

Genbank Nucleotide ID

 BAH11465.1; EAW99738.1 

Protein Name

 Chaperonin containing TCP1, subunit 7 (Eta), isoform CRA_a 

Protein Synonyms/Alias

 T-complex protein 1 subunit eta; cDNA FLJ59454, highly similar to T-complex protein 1 subunit eta 

Gene Name

 CCT7 

Gene Synonyms/Alias

 hCG_1997313 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
29	EQRKLLEKCAMTALS	acetylation	[1]
29	EQRKLLEKCAMTALS	ubiquitination	[2, 3]
38	AMTALSSKLISQQKA	ubiquitination	[2, 3, 4, 5]
44	SKLISQQKAFFAKMV	acetylation	[3]
44	SKLISQQKAFFAKMV	ubiquitination	[2, 3, 6]
71	LKMIGIKKVQGGALE	ubiquitination	[2]
89	LVAGVAFKKTFSYAG	ubiquitination	[3, 4, 7, 8]
90	VAGVAFKKTFSYAGF	ubiquitination	[2, 3, 4, 5, 6]
102	AGFEMQPKKYHNPKI	ubiquitination	[2, 3]
103	GFEMQPKKYHNPKIA	ubiquitination	[2, 3]
122	ELELKAEKDNAEIRV	acetylation	[3]
122	ELELKAEKDNAEIRV	ubiquitination	[9]
159	KIHHSGAKVVLSKLP	acetylation	[3]
159	KIHHSGAKVVLSKLP	ubiquitination	[2, 3]
164	GAKVVLSKLPIGDVA	acetylation	[1]
164	GAKVVLSKLPIGDVA	ubiquitination	[2, 3, 4, 6, 7, 8]
192	RVPEEDLKRTMMACG	acetylation	[3]
192	RVPEEDLKRTMMACG	ubiquitination	[2, 3, 4, 5]
238	NFFTGCPKAKTCTFI	ubiquitination	[2, 3, 4, 5, 6]
240	FTGCPKAKTCTFILR	ubiquitination	[2, 3, 5]
273	MIVRRAIKNDSVVAG	ubiquitination	[4]
290	AIEMELSKYLRDYSR	ubiquitination	[2, 3, 4, 6, 7, 8]
302	YSRTIPGKQQLLIGA	ubiquitination	[2, 3, 6]
312	LLIGAYAKALEIIPR	ubiquitination	[2, 3, 4, 6, 7, 8]
335	DATNILNKLRARHAQ	acetylation	[1, 3]
335	DATNILNKLRARHAQ	ubiquitination	[2, 3, 4, 5, 6, 7, 9]
393	VSVDETIKNPRSTVD	ubiquitination	[2, 4, 9]

Reference

 [1] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [5] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [6] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [7] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [8] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [9] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931] 

Functional Description

  

Sequence Annotation

  

Keyword

 ATP-binding; Chaperone; Complete proteome; Cytoplasm; Nucleotide-binding; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 415 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IDA:HPA.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0006457; P:protein folding; IEA:InterPro. 

Interpro

 IPR012720; Chap_CCT_eta.
 IPR017998; Chaperone_TCP-1.
 IPR002423; Cpn60/TCP-1.
 IPR027409; GroEL-like_apical_dom.
 IPR027413; GROEL-like_equatorial.
 IPR027410; TCP-1-like_intermed. 

Pfam

 PF00118; Cpn60_TCP1 

SMART

  

PROSITE

  

PRINTS

 PR00304; TCOMPLEXTCP1.