CPLM-011528

Genbank Nucleotide ID

Bcl-2-like protein 1

Protein Synonyms/Alias

Bcl2-L-1; Apoptosis regulator Bcl-X

Homo sapiens (Human)

Position	Peptide	Type	References
16	VVDFLSYKLSQKGYS	ubiquitination	[1, 2, 3]
87	VIPMAAVKQALREAG	ubiquitination	[1, 2, 3, 4, 5, 6]

[1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
[2] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
[3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
Chen Z, Zhou Y, Song J, Zhang Z.
Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
[4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
[5] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
[6] Integrated proteomic analysis of post-translational modifications by serial enrichment.
Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]

Functional Description

Potent inhibitor of cell death. Inhibits activation of caspases (By similarity). Appears to regulate cell death by blocking the voltage-dependent anion channel (VDAC) by binding to it and preventing the release of the caspase activator, CYC1, from the mitochondrial membrane. Also acts as a regulator of G2 checkpoint and progression to cytokinesis during mitosis.

MOTIF 4 24 BH4.
MOTIF 86 100 BH3.
MOTIF 129 148 BH1.
MOTIF 180 195 BH2.
MOD_RES 49 49 Phosphoserine; by PLK3.
MOD_RES 62 62 Phosphoserine; by CDK1.

3D-structure; Alternative splicing; Apoptosis; Complete proteome; Cytoplasm; Cytoskeleton; Membrane; Mitochondrion; Nucleus; Phosphoprotein; Reference proteome; Transmembrane; Transmembrane helix.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0097136; C:Bcl-2 family protein complex; IDA:UniProtKB.
GO:0005813; C:centrosome; IDA:UniProtKB.
GO:0005829; C:cytosol; IEA:Compara.
GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
GO:0005741; C:mitochondrial outer membrane; NAS:UniProtKB.
GO:0031965; C:nuclear membrane; IEA:UniProtKB-SubCell.
GO:0008283; P:cell proliferation; IEA:Compara.
GO:0060154; P:cellular process regulating host cell cycle in response to virus; IEA:Compara.
GO:0071312; P:cellular response to alkaloid; IEA:Compara.
GO:0071230; P:cellular response to amino acid stimulus; IEA:Compara.
GO:0071480; P:cellular response to gamma radiation; IEA:Compara.
GO:0000910; P:cytokinesis; IMP:UniProtKB.
GO:0009566; P:fertilization; IEA:Compara.
GO:0007281; P:germ cell development; IEA:Compara.
GO:0040007; P:growth; IEA:Compara.
GO:0001701; P:in utero embryonic development; IEA:Compara.
GO:0045087; P:innate immune response; TAS:Reactome.
GO:0097193; P:intrinsic apoptotic signaling pathway; TAS:Reactome.
GO:0008584; P:male gonad development; IEA:Compara.
GO:0007093; P:mitotic cell cycle checkpoint; IMP:UniProtKB.
GO:2000811; P:negative regulation of anoikis; IMP:UniProtKB.
GO:0043066; P:negative regulation of apoptotic process; IDA:UniProtKB.
GO:0010507; P:negative regulation of autophagy; TAS:UniProtKB.
GO:0090005; P:negative regulation of establishment of protein localization to plasma membrane; IDA:BHF-UCL.
GO:1900118; P:negative regulation of execution phase of apoptosis; IDA:UniProtKB.
GO:2001243; P:negative regulation of intrinsic apoptotic signaling pathway; IDA:BHF-UCL.
GO:0043524; P:negative regulation of neuron apoptotic process; IEA:Compara.
GO:0090201; P:negative regulation of release of cytochrome c from mitochondria; IC:BHF-UCL.
GO:0035872; P:nucleotide-binding domain, leucine rich repeat containing receptor signaling pathway; TAS:Reactome.
GO:0001541; P:ovarian follicle development; IEA:Compara.
GO:0043065; P:positive regulation of apoptotic process; IEA:Compara.
GO:0008284; P:positive regulation of cell proliferation; IEA:Compara.
GO:2001244; P:positive regulation of intrinsic apoptotic signaling pathway; TAS:Reactome.
GO:0046902; P:regulation of mitochondrial membrane permeability; IDA:HGNC.
GO:0051881; P:regulation of mitochondrial membrane potential; IDA:HGNC.
GO:0001836; P:release of cytochrome c from mitochondria; IDA:HGNC.
GO:0046898; P:response to cycloheximide; IEA:Compara.
GO:0034097; P:response to cytokine stimulus; IDA:MGI.
GO:0007283; P:spermatogenesis; IEA:Compara.

IPR013279; Apop_reg_BclX.
IPR002475; Bcl2-like.
IPR004725; Bcl2/BclX.
IPR020717; Bcl2_BH1_motif_CS.
IPR020726; Bcl2_BH2_motif_CS.
IPR020728; Bcl2_BH3_motif_CS.
IPR003093; Bcl2_BH4.
IPR020731; Bcl2_BH4_motif_CS.
IPR026298; Blc2_fam.

PF00452; Bcl-2
PF02180; BH4

PS50062; BCL2_FAMILY
PS01080; BH1
PS01258; BH2
PS01259; BH3
PS01260; BH4_1
PS50063; BH4_2

PR01864; APOPREGBCLX.
PR01862; BCL2FAMILY.