CPLM-019457

Genbank Nucleotide ID

Melanoma-associated antigen G1

Protein Synonyms/Alias

Hepatocellular carcinoma-associated protein 4; MAGE-G1 antigen; Necdin-like protein 2

Homo sapiens (Human)

Position	Peptide	Type	References
83	AVGPRSQKQLELKVS	ubiquitination	[1, 2]
113	IKRADILKHVIGDYK	ubiquitination	[3, 4]
128	DIFPDLFKRAAERLQ	ubiquitination	[3, 4]
141	LQYVFGYKLVELEPK	ubiquitination	[3, 4]
212	LGVYPTKKHLIFGDP	ubiquitination	[5]
221	LIFGDPKKLITEDFV	ubiquitination	[5]

[1] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
[2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
[3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
[4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
Chen Z, Zhou Y, Song J, Zhang Z.
Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
[5] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]

Functional Description

Component of the SMC5-SMC6 complex, a complex involved in repair of DNA double-strand breaks by homologous recombination. The complex may promote sister chromatid homologous recombination by recruiting the SMC1-SMC3 cohesin complex to double-strand breaks. The complex is required for telomere maintenance via recombination in ALT (alternative lengthening of telomeres) cell lines and mediates sumoylation of shelterin complex (telosome) components which is proposed to lead to shelterin complex disassembly in ALT-associated PML bodies (APBs). In vitro enhances ubiquitin ligase activity of NSMCE1. Proposed to act through recruitment and/or stabilization of the Ubl-conjugating enzyme (E2) at the E3:substrate complex. May be a growth suppressor that facilitates the entry of the cell into cell cycle arrest.

DOMAIN 85 285 MAGE.
REGION 78 304 Interaction with NSMCE1.
MOD_RES 57 57 Phosphoserine.
MOD_RES 64 64 Phosphoserine.

3D-structure; Chromosome; Complete proteome; Cytoplasm; DNA damage; DNA recombination; DNA repair; Growth regulation; Nucleus; Phosphoprotein; Reference proteome; Telomere; Tumor antigen; Ubl conjugation pathway.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0000781; C:chromosome, telomeric region; IEA:UniProtKB-SubCell.
GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
GO:0030915; C:Smc5-Smc6 complex; IDA:UniProtKB.
GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
GO:0031398; P:positive regulation of protein ubiquitination; IDA:UniProtKB.
GO:0040008; P:regulation of growth; IEA:UniProtKB-KW.
GO:0006355; P:regulation of transcription, DNA-dependent; IEA:Compara.