CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-016525
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 NADP-dependent malic enzyme, mitochondrial 
Protein Synonyms/Alias
 NADP-ME; Malic enzyme 3 
Gene Name
 Me3 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
119TLQDRNEKLFYRVLTacetylation[1]
Reference
 [1] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441] 
Functional Description
  
Sequence Annotation
 ACT_SITE 137 137 Proton donor (By similarity).
 ACT_SITE 208 208 Proton acceptor (By similarity).
 METAL 280 280 Divalent metal cation (By similarity).
 METAL 281 281 Divalent metal cation (By similarity).
 METAL 304 304 Divalent metal cation (By similarity).
 BINDING 190 190 NADP (By similarity).
 BINDING 304 304 NADP (By similarity).
 BINDING 443 443 NADP (By similarity).  
Keyword
 Complete proteome; Direct protein sequencing; Metal-binding; Mitochondrion; NADP; Oxidoreductase; Reference proteome; Transit peptide. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 604 AA 
Protein Sequence
MGAALGTGAR LTSVPRIACS SLRRQAPSAP AQGCHSKSGP PRPVPLKKRG YDVTRNPHLN 60
KGMAFTLEER LQLGIHGLIP PCFLSQDVQL LRIMRYYENQ QSDLDKYIIL MTLQDRNEKL 120
FYRVLTSDVE KFMPIVYTPT VGLACQHYGL TFRRPRGLFI TIHDKGHIAT MLNSWPEDNI 180
KAVVVTDGER ILGLGDLGCY GMGIPVGKLA LYTACGGVNP QQCLPVLLDV GTNNEELLRD 240
PLYIGLKHQR VRGEEYDDLL DEFMQAVTDK FGINCLIQFE DFANANAFRL LNKYRNKYCM 300
FNDDIQGTAS VAVAGILAAL RITKNRLSNH VFVFQGAGEA AMGIAHLLVM ALEKEGIPKT 360
EAIKKIWMVD SKGLIVKGRS HLNHEKEMFA QDHPEVNSLE EVVRLVKPTA IIGVAAIAGA 420
FTEQILRDMA SFHERPIVFA LSNPTSKAEC TAEKCYRVTE GRGIFASGSP FKSVTLEDGR 480
TFTPGQGNNA YVFPGVALGV IAGGIRHIPD EIFLLTAEQI AQEVSEQHLS QGRLYPPLST 540
IRDVSLRIAV KVLDYAYKHN LASYYPEPKD KEAFVKSLIY TPDYDSFSLD TYSWPKEAMS 600
VQKV 604 
Gene Ontology
 GO:0005759; C:mitochondrial matrix; IEA:UniProtKB-SubCell.
 GO:0005739; C:mitochondrion; IDA:MGI.
 GO:0004473; F:malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+) activity; ISS:UniProtKB.
 GO:0016619; F:malate dehydrogenase (oxaloacetate-decarboxylating) activity; IEA:InterPro.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0051287; F:NAD binding; IEA:InterPro.
 GO:0006108; P:malate metabolic process; ISS:UniProtKB.
 GO:0006090; P:pyruvate metabolic process; ISS:UniProtKB. 
Interpro
 IPR015884; Malic_enzyme_CS.
 IPR012301; Malic_N.
 IPR012302; Malic_NAD-bd.
 IPR001891; Malic_OxRdtase.
 IPR016040; NAD(P)-bd_dom. 
Pfam
 PF00390; malic
 PF03949; Malic_M 
SMART
 SM00919; Malic_M 
PROSITE
 PS00331; MALIC_ENZYMES 
PRINTS
 PR00072; MALOXRDTASE.