UniProt Accession

 H15_MOUSE; P43276; Q9CRM8 

Genbank Protein ID

 Z46227; U62922; AY158904; AK020117 

Genbank Nucleotide ID

 CAA86299.1; AAB05798.1; AAO06215.1; BAB32001.1 

Protein Name

 Histone H1.5 

Protein Synonyms/Alias

 H1 VAR.5; H1b 

Gene Name

 Hist1h1b 

Gene Synonyms/Alias

 H1f5 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
17	AAPAPVEKSPAKKKT	acetylation	[1, 2, 3]
17	AAPAPVEKSPAKKKT	succinylation	[3]
34	KAGAAKRKATGPPVS	acetylation	[3]
34	KAGAAKRKATGPPVS	succinylation	[3]
46	PVSELITKAVSASKE	acetylation	[2, 3, 4]
46	PVSELITKAVSASKE	ubiquitination	[5]
63	GVSLPALKKALAAGG	acetylation	[3]
63	GVSLPALKKALAAGG	succinylation	[3]
64	VSLPALKKALAAGGY	acetylation	[3]
64	VSLPALKKALAAGGY	succinylation	[3]
75	AGGYDVEKNNSRIKL	acetylation	[2, 3]
75	AGGYDVEKNNSRIKL	ubiquitination	[5]
90	GLKSLVSKGTLVQTK	acetylation	[1, 6]
158	KTPKKAKKPAAAGVK	acetylation	[6]
165	KPAAAGVKKVAKSPK	acetylation	[6]

Reference

 [1] Comprehensive mapping of post-translational modifications on synaptic, nuclear, and histone proteins in the adult mouse brain.
 Tweedie-Cullen RY, Reck JM, Mansuy IM.
 J Proteome Res. 2009 Nov;8(11):4966-82. [PMID: 19737024]
 [2] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [3] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [4] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [5] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [6] Mass spectrometric mapping of linker histone H1 variants reveals multiple acetylations, methylations, and phosphorylation as well as differences between cell culture and tissue.
 Wisniewski JR, Zougman A, Krüger S, Mann M.
 Mol Cell Proteomics. 2007 Jan;6(1):72-87. [PMID: 17043054] 

Functional Description

 Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation (By similarity). 

Sequence Annotation

 DOMAIN 36 109 H15.
 MOD_RES 2 2 N-acetylserine.
 MOD_RES 2 2 Phosphoserine (By similarity).
 MOD_RES 4 4 Phosphothreonine.
 MOD_RES 18 18 Phosphoserine.
 MOD_RES 27 27 N6-methyllysine (By similarity).
 MOD_RES 36 36 Phosphothreonine (By similarity).
 MOD_RES 135 135 Phosphothreonine (By similarity).
 MOD_RES 165 165 N6-acetyllysine (By similarity).
 MOD_RES 170 170 Phosphoserine (By similarity).  

Keyword

 Acetylation; Chromosome; Complete proteome; DNA-binding; Methylation; Nucleus; Phosphoprotein; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 223 AA 

Protein Sequence

Gene Ontology

 GO:0000786; C:nucleosome; IEA:InterPro.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0003677; F:DNA binding; IDA:MGI.
 GO:0007517; P:muscle organ development; IPI:MGI.
 GO:0006334; P:nucleosome assembly; IEA:InterPro. 

Interpro

 IPR005818; Histone_H1/H5.
 IPR005819; Histone_H5.
 IPR011991; WHTH_DNA-bd_dom. 

Pfam

 PF00538; Linker_histone 

SMART

 SM00526; H15 

PROSITE

 PS51504; H15 

PRINTS

 PR00624; HISTONEH5.