UniProt Accession

 PSB5_YEAST; P30656; D6W4A1 

Genbank Protein ID

 M96667; X68662; U32445; Z28348; BK006949 

Genbank Nucleotide ID

 AAA34906.1; CAA48628.1; AAB68073.1; CAA82203.1; DAA11517.1 

Protein Name

 Proteasome subunit beta type-5 

Protein Synonyms/Alias

 Macropain subunit PRE2; Multicatalytic endopeptidase complex subunit PRE2; Proteasome component PRE2; Proteinase YSCE subunit PRE2 

Gene Name

 PRE2 

Gene Synonyms/Alias

 DOA3; PRG1; YPR103W; P8283.10 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
107	WVASQTVKKVIEINP	ubiquitination	[1]
156	ISVAAASKILSNLVY	ubiquitination	[1]
182	MICGYTRKEGPTIYY	ubiquitination	[1]
276	GELFWKVKEEEGSFN	ubiquitination	[1]

Reference

 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 

Functional Description

 The proteasome degrades poly-ubiquitinated proteins in the cytoplasm and in the nucleus. It is essential for the regulated turnover of proteins and for the removal of misfolded proteins. The proteasome is a multicatalytic proteinase complex that is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. It has an ATP-dependent proteolytic activity. This unit is responsible of the chymotrypsin-like activity of the proteasome and is one of the principal target of the proteasome inhibitor bortezomib. 

Sequence Annotation

 ACT_SITE 76 76 Nucleophile.
 BINDING 124 124 Bortezomib; via amide nitrogen.  

Keyword

 3D-structure; Complete proteome; Cytoplasm; Direct protein sequencing; Hydrolase; Nucleus; Protease; Proteasome; Reference proteome; Threonine protease; Zymogen. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 287 AA 

Protein Sequence

Gene Ontology

 GO:0005789; C:endoplasmic reticulum membrane; IC:SGD.
 GO:0005634; C:nucleus; IC:SGD.
 GO:0019774; C:proteasome core complex, beta-subunit complex; IDA:SGD.
 GO:0034515; C:proteasome storage granule; IC:SGD.
 GO:0004175; F:endopeptidase activity; IMP:SGD.
 GO:0004298; F:threonine-type endopeptidase activity; IEA:UniProtKB-KW.
 GO:0043161; P:proteasomal ubiquitin-dependent protein catabolic process; IDA:SGD.
 GO:0010499; P:proteasomal ubiquitin-independent protein catabolic process; IDA:SGD.
 GO:0080129; P:proteasome core complex assembly; IMP:SGD. 

Interpro

 IPR000243; Pept_T1A_subB.
 IPR016050; Proteasome_bsu_CS.
 IPR001353; Proteasome_sua/b.
 IPR023333; Proteasome_suB-type. 

Pfam

 PF00227; Proteasome 

SMART

  

PROSITE

 PS00854; PROTEASOME_B_1
 PS51476; PROTEASOME_B_2 

PRINTS

 PR00141; PROTEASOME.