CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-004115
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 TATA-box-binding protein 
Protein Synonyms/Alias
 TATA sequence-binding protein; TBP; TATA-binding factor; TATA-box factor; Transcription factor D; Transcription initiation factor TFIID TBP subunit 
Gene Name
 SPT15 
Gene Synonyms/Alias
 BTF1; TBP1; YER148W 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
15KEFKEANKIVFDPNTacetylation[1]
127KMVVTGAKSEDDSKLubiquitination[2]
133AKSEDDSKLASRKYAubiquitination[2]
151QKIGFAAKFTDFKIQubiquitination[2]
167IVGSCDVKFPIRLEGubiquitination[2]
Reference
 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [2] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 
Functional Description
 General transcription factor that functions at the core of the DNA-binding general transcription factor complex TFIID. Binding of TFIID to a promoter (with or without TATA element) is the initial step in preinitiation complex (PIC) formation. TFIID plays a key role in the regulation of gene expression by RNA polymerase II through different activities such as transcription activator interaction, core promoter recognition and selectivity, TFIIA and TFIIB interaction, chromatin modification (histone acetylation by TAF1), facilitation of DNA opening and initiation of transcription. 
Sequence Annotation
 REPEAT 67 143 1.
 REPEAT 157 234 2.  
Keyword
 3D-structure; Complete proteome; Direct protein sequencing; DNA-binding; Nucleus; Reference proteome; Repeat; Transcription. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 240 AA 
Protein Sequence
MADEERLKEF KEANKIVFDP NTRQVWENQN RDGTKPATTF QSEEDIKRAA PESEKDTSAT 60
SGIVPTLQNI VATVTLGCRL DLKTVALHAR NAEYNPKRFA AVIMRIREPK TTALIFASGK 120
MVVTGAKSED DSKLASRKYA RIIQKIGFAA KFTDFKIQNI VGSCDVKFPI RLEGLAFSHG 180
TFSSYEPELF PGLIYRMVKP KIVLLIFVSG KIVLTGAKQR EEIYQAFEAI YPVLSEFRKM 240 
Gene Ontology
 GO:0005669; C:transcription factor TFIID complex; IDA:SGD.
 GO:0000126; C:transcription factor TFIIIB complex; IDA:SGD.
 GO:0003682; F:chromatin binding; IDA:SGD.
 GO:0008301; F:DNA binding, bending; IDA:SGD.
 GO:0001179; F:RNA polymerase I transcription factor binding; IDA:SGD.
 GO:0001186; F:RNA polymerase I transcription factor recruiting transcription factor activity; IDA:SGD.
 GO:0001102; F:RNA polymerase II activating transcription factor binding; IDA:SGD.
 GO:0000979; F:RNA polymerase II core promoter sequence-specific DNA binding; IDA:SGD.
 GO:0001075; F:RNA polymerase II core promoter sequence-specific DNA binding transcription factor activity involved in preinitiation complex assembly; IC:SGD.
 GO:0001016; F:RNA polymerase III regulatory region DNA binding; IDA:SGD.
 GO:0001026; F:TFIIIB-type transcription factor activity; IC:SGD.
 GO:0070898; P:RNA polymerase III transcriptional preinitiation complex assembly; IDA:SGD.
 GO:0042790; P:transcription of nuclear large rRNA transcript from RNA polymerase I promoter; IMP:SGD.
 GO:0070893; P:transposon integration; IDA:SGD. 
Interpro
 IPR012295; Beta2_adaptin/TBP_C_dom.
 IPR000814; TBP. 
Pfam
 PF00352; TBP 
SMART
  
PROSITE
 PS00351; TFIID 
PRINTS
 PR00686; TIFACTORIID.