UniProt Accession

 SLAP2_HUMAN; Q9H6Q3; A8K648; E1P5U1; E1P5U2; Q5TH27; Q5TH28; Q8WY18; Q96QI4; Q9H135 

Genbank Protein ID

 AF326353; AF290985; AF290986; AK025645; AK291513; AL031662; AL050318; AL031662; AL050318; AL050318; AL031662; AL050318; AL031662; CH471077; CH471077; CH471077; CH471077; BC042041 

Genbank Nucleotide ID

 AAL29204.1; AAL38197.1; AAL38198.1; BAB15201.1; BAF84202.1; CAI21397.1; CAI21397.1; CAI21398.1; CAI21398.1; CAI23504.1; CAI23504.1; CAI23505.1; CAI23505.1; EAW76116.1; EAW76117.1; EAW76118.1; EAW76119.1; AAH42041.1 

Protein Name

 Src-like-adapter 2 

Protein Synonyms/Alias

 Modulator of antigen receptor signaling; MARS; Src-like adapter protein 2; SLAP-2 

Gene Name

 SLA2 

Gene Synonyms/Alias

 C20orf156; SLAP2 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
34	TMEAERSKATAVALG	ubiquitination	[1, 2]
103	YEGLSREKAEELLLL	ubiquitination	[1]
202	RAGPLPGKDIPLPVT	ubiquitination	[1, 2, 3]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [3] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724] 

Functional Description

 Adapter protein, which negatively regulates T-cell receptor (TCR) signaling. Inhibits T-cell antigen-receptor induced activation of nuclear factor of activated T-cells. May act by linking signaling proteins such as ZAP70 with CBL, leading to a CBL dependent degradation of signaling proteins. 

Sequence Annotation

 DOMAIN 32 92 SH3.
 DOMAIN 94 191 SH2.
 REGION 195 261 SLA C-terminal.
 LIPID 2 2 N-myristoyl glycine.  

Keyword

 Alternative initiation; Alternative splicing; Cell membrane; Complete proteome; Cytoplasm; Cytoplasmic vesicle; Lipoprotein; Membrane; Myristate; Phosphoprotein; Polymorphism; Reference proteome; SH2 domain; SH3 domain. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 261 AA 

Protein Sequence

Gene Ontology

 GO:0016023; C:cytoplasmic membrane-bounded vesicle; IEA:UniProtKB-SubCell.
 GO:0010008; C:endosome membrane; NAS:UniProtKB.
 GO:0005770; C:late endosome; IEA:Compara.
 GO:0005886; C:plasma membrane; TAS:UniProtKB.
 GO:0005070; F:SH3/SH2 adaptor activity; IDA:UniProtKB.
 GO:0050851; P:antigen receptor-mediated signaling pathway; TAS:UniProtKB.
 GO:0019724; P:B cell mediated immunity; TAS:UniProtKB.
 GO:0030522; P:intracellular receptor signaling pathway; TAS:UniProtKB.
 GO:0050869; P:negative regulation of B cell activation; TAS:UniProtKB.
 GO:0050849; P:negative regulation of calcium-mediated signaling; IDA:UniProtKB.
 GO:0000122; P:negative regulation of transcription from RNA polymerase II promoter; IDA:UniProtKB.
 GO:0042110; P:T cell activation; TAS:UniProtKB. 

Interpro

 IPR000980; SH2.
 IPR001452; SH3_domain. 

Pfam

 PF00017; SH2
 PF00018; SH3_1 

SMART

 SM00252; SH2
 SM00326; SH3 

PROSITE

 PS50001; SH2
 PS50002; SH3 

PRINTS

 PR00401; SH2DOMAIN.