CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-022253
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 WD repeat-containing protein 70 
Protein Synonyms/Alias
  
Gene Name
 WDR70 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
33GFTGFGKKARTFDLEubiquitination[1]
296GSWHPKIKGEFMTCSubiquitination[1]
318WEVENPKKQKSVFKPubiquitination[1]
332PRTMQGKKVIPTTCTubiquitination[1]
403RGGDDSLKLWDIRQFubiquitination[1, 2]
452QRGCGSGKLVFFERRacetylation[3, 4]
452QRGCGSGKLVFFERRubiquitination[1]
482VRCLWHPKLNQIMVGubiquitination[1]
496GTGNGLAKVYYDPNKubiquitination[1]
509NKSQRGAKLCVVKTQacetylation[4]
509NKSQRGAKLCVVKTQubiquitination[1]
514GAKLCVVKTQRKAKQubiquitination[1]
520VKTQRKAKQAETLTQacetylation[3]
611EAILRHAKAAEDSPYacetylation[5]
626WVSPAYSKTQPKTMFacetylation[5]
626WVSPAYSKTQPKTMFubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [4] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [5] Regulation of cellular metabolism by protein lysine acetylation.
 Zhao S, Xu W, Jiang W, Yu W, Lin Y, Zhang T, Yao J, Zhou L, Zeng Y, Li H, Li Y, Shi J, An W, Hancock SM, He F, Qin L, Chin J, Yang P, Chen X, Lei Q, Xiong Y, Guan KL.
 Science. 2010 Feb 19;327(5968):1000-4. [PMID: 20167786
Functional Description
  
Sequence Annotation
 REPEAT 180 219 WD 1.
 REPEAT 227 268 WD 2.
 REPEAT 281 321 WD 3.
 REPEAT 330 369 WD 4.
 REPEAT 376 415 WD 5.
 REPEAT 421 466 WD 6.
 REPEAT 469 508 WD 7.
 MOD_RES 452 452 N6-acetyllysine.
 MOD_RES 621 621 Phosphoserine.
 MOD_RES 638 638 Phosphoserine.  
Keyword
 Acetylation; Complete proteome; Phosphoprotein; Reference proteome; Repeat; WD repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 654 AA 
Protein Sequence
MERSGPSEVT GSDASGPDPQ LAVTMGFTGF GKKARTFDLE AMFEQTRRTA VERSRKTLEA 60
REKEEEMNRE KELRRQNEDI EPTSSRSNVV RDCSKSSSRD TSSSESEQSS DSSDDELIGP 120
PLPPKMVGKP VNFMEEDILG PLPPPLNEEE EEAEEEEEEE EEEENPVHKI PDSHEITLKH 180
GTKTVSALGL DPSGARLVTG GYDYDVKFWD FAGMDASFKA FRSLQPCECH QIKSLQYSNT 240
GDMILVVSGS SQAKVIDRDG FEVMECIKGD QYIVDMANTK GHTAMLHTGS WHPKIKGEFM 300
TCSNDATVRT WEVENPKKQK SVFKPRTMQG KKVIPTTCTY SRDGNLIAAA CQNGSIQIWD 360
RNLTVHPKFH YKQAHDSGTD TSCVTFSYDG NVLASRGGDD SLKLWDIRQF NKPLFSASGL 420
PTMFPMTDCC FSPDDKLIVT GTSIQRGCGS GKLVFFERRT FQRVYEIDIT DASVVRCLWH 480
PKLNQIMVGT GNGLAKVYYD PNKSQRGAKL CVVKTQRKAK QAETLTQDYI ITPHALPMFR 540
EPRQRSTRKQ LEKDRLDPLK SHKPEPPVAG PGRGGRVGTH GGTLSSYIVK NIALDKTDDS 600
NPREAILRHA KAAEDSPYWV SPAYSKTQPK TMFAQVESDD EEAKNEPEWK KRKI 654 
Gene Ontology
  
Interpro
 IPR015943; WD40/YVTN_repeat-like_dom.
 IPR001680; WD40_repeat.
 IPR019775; WD40_repeat_CS.
 IPR017986; WD40_repeat_dom. 
Pfam
 PF00400; WD40 
SMART
 SM00320; WD40 
PROSITE
 PS00678; WD_REPEATS_1
 PS50082; WD_REPEATS_2
 PS50294; WD_REPEATS_REGION 
PRINTS