CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-037260
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 Splicing factor 3A subunit 1 
Protein Synonyms/Alias
  
Gene Name
 SF3A1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
55EVRNIVDKTASFVARacetylation[1]
55EVRNIVDKTASFVARubiquitination[2, 3]
80QNEINNPKFNFLNPNacetylation[4]
80QNEINNPKFNFLNPNubiquitination[2]
123FLTQLMQKEQRNYQFubiquitination[2]
158TKILIPPKGLFSKLKacetylation[1]
158TKILIPPKGLFSKLKubiquitination[2]
186CYRVEWAKFQERERKubiquitination[2]
334DYDPKASKPLPPAPAubiquitination[5, 6]
354VSPITGEKIPASKMQubiquitination[1, 2, 3, 5, 6]
359GEKIPASKMQEHMRIacetylation[1]
359GEKIPASKMQEHMRIubiquitination[3]
384RDRSIREKQSDDEVYubiquitination[3, 5]
402LDIESSLKQLAERRTubiquitination[2, 5]
421VEETAIGKKIGEEEIacetylation[1, 4]
421VEETAIGKKIGEEEIubiquitination[5]
422EETAIGKKIGEEEIQubiquitination[3]
430IGEEEIQKPEEKVTWacetylation[1]
643NKGPVSIKVQVPNMQubiquitination[2]
Reference
 [1] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [5] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [6] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 728 AA 
Protein Sequence
MPAGPVQAVP PPPPVPTEPK QPTEEEASSK EDSAPSKPVV GIIYPPPEVR NIVDKTASFV 60
ARNGPEFEAR IRQNEINNPK FNFLNPNDPY HAYYRHKVSE FKEGKAQFVA RNGRQFLTQL 120
MQKEQRNYQF DFLRPQHSLF NYFTKLVEQY TKILIPPKGL FSKLKKEAEN PREVLDQVCY 180
RVEWAKFQER ERKKEEEEKE KERVAYAQID WHDFVVVETV DFQPNEQGNF PPPTTPEELG 240
ARILIQERYE KFGESEEVEM EVESDEEDDK QEKAEEPPSQ LDQDTQVQDM DEGSDDEEEG 300
QKVPPPPETP MPPPLPPTPD QVIVRKDYDP KASKPLPPAP APDEYLVSPI TGEKIPASKM 360
QEHMRIGLLD PRWLEQRDRS IREKQSDDEV YAPGLDIESS LKQLAERRTD IFGVEETAIG 420
KKIGEEEIQK PEEKVTWDGH SGSMARTQQA AQANITLQEQ IEAIHKAKGL VPEDDTKEKI 480
GPSKPNEIPQ QPPPPSSATN IPSSAPPITS VPRPPTMPPP VRTTVVSAVP VMPRPPMASV 540
VRLPPGSVIA PMPPIIHAPR INVVPMPPSA PPIMAPRPPP MIVPTAFVPA PPVAPVPAPA 600
PMPPVHPPPP MEDEPTSKKL KTEDSLMPEE EFLRRNKGPV SIKVQVPNMQ DKTEWKLNGQ 660
VLVFTLPLTD QVSVIKVKIH EATGMPAGKQ KLQYEGIFIK DSNSLAYYNM ANGAVIHLAL 720
KERGGRKK 728 
Gene Ontology
 GO:0005634; C:nucleus; IDA:HPA.
 GO:0003723; F:RNA binding; IEA:InterPro.
 GO:0006396; P:RNA processing; IEA:InterPro. 
Interpro
 IPR022030; PRP21-like.
 IPR000061; Surp.
 IPR000626; Ubiquitin.
 IPR019955; Ubiquitin_supergroup. 
Pfam
 PF12230; PRP21_like_P
 PF01805; Surp
 PF00240; ubiquitin 
SMART
 SM00648; SWAP
 SM00213; UBQ 
PROSITE
 PS50128; SURP
 PS50053; UBIQUITIN_2 
PRINTS