UniProt Accession

 GNP1_YEAST; P48813; D6VTD0 

Genbank Protein ID

 U21643; U33057; BK006938 

Genbank Nucleotide ID

 AAB48002.1; AAB64950.1; DAA12340.1 

Protein Name

 High-affinity glutamine permease 

Protein Synonyms/Alias

  

Gene Name

 GNP1 

Gene Synonyms/Alias

 YDR508C; D9719.14 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
34	DVSHYEMKEIQPKEK	ubiquitination	[1, 2, 3, 4, 5]
39	EMKEIQPKEKQIGSI	ubiquitination	[4]
41	KEIQPKEKQIGSIEP	ubiquitination	[1, 2, 3, 4, 5]
57	NEVEYFEKTVEKTIE	ubiquitination	[4, 5]
61	YFEKTVEKTIENMEY	ubiquitination	[4, 5]
109	GTTPISTKDSSSQLD	ubiquitination	[5]
122	LDNELNRKSSYITVD	ubiquitination	[5]
132	YITVDGIKQSPQEQE	ubiquitination	[1, 2, 3, 5]
141	SPQEQEQKQENLKKS	ubiquitination	[5]
373	SEQSNPRKAIPSAAK	ubiquitination	[5]
465	RILMSLAKQGNAPKC	ubiquitination	[5]
471	AKQGNAPKCFDYIDR	ubiquitination	[5]

Reference

 [1] A proteomics approach to understanding protein ubiquitination.
 Peng J, Schwartz D, Elias JE, Thoreen CC, Cheng D, Marsischky G, Roelofs J, Finley D, Gygi SP.
 Nat Biotechnol. 2003 Aug;21(8):921-6. [PMID: 12872131]
 [2] Computational identification of ubiquitylation sites from protein sequences.
 Tung CW, Ho SY.
 BMC Bioinformatics. 2008 Jul 15;9:310. [PMID: 18625080]
 [3] Identification, analysis, and prediction of protein ubiquitination sites.
 Radivojac P, Vacic V, Haynes C, Cocklin RR, Mohan A, Heyen JW, Goebl MG, Iakoucheva LM.
 Proteins. 2010 Feb 1;78(2):365-80. [PMID: 19722269]
 [4] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
 Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
 Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047]
 [5] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 

Functional Description

 High affinity transport of glutamine. Also transport Leu, Ser, Thr, Cys, Met and Asn. 

Sequence Annotation

 MOD_RES 29 29 Phosphoserine.
 MOD_RES 113 113 Phosphoserine.
 MOD_RES 124 124 Phosphoserine.
 MOD_RES 127 127 Phosphothreonine.
 CROSSLNK 34 34 Glycyl lysine isopeptide (Lys-Gly)
 CROSSLNK 41 41 Glycyl lysine isopeptide (Lys-Gly)
 CROSSLNK 132 132 Glycyl lysine isopeptide (Lys-Gly)  

Keyword

 Amino-acid transport; Complete proteome; Isopeptide bond; Membrane; Mitochondrion; Phosphoprotein; Reference proteome; Transmembrane; Transmembrane helix; Transport; Ubl conjugation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 663 AA 

Protein Sequence

Gene Ontology

 GO:0016021; C:integral to membrane; ISM:SGD.
 GO:0031966; C:mitochondrial membrane; IEA:UniProtKB-SubCell.
 GO:0005739; C:mitochondrion; IDA:SGD.
 GO:0005886; C:plasma membrane; ISS:SGD.
 GO:0015193; F:L-proline transmembrane transporter activity; IGI:SGD. 

Interpro

 IPR004841; AA-permease_dom.
 IPR002293; AA/rel_permease1.
 IPR004762; Amino_acid_permease_fungi.
 IPR004840; Amoino_acid_permease_CS. 

Pfam

 PF00324; AA_permease 

SMART

  

PROSITE

 PS00218; AMINO_ACID_PERMEASE_1 

PRINTS