UniProt Accession

 PIWL4_HUMAN; Q7Z3Z4; B4DEG5; Q68CZ4; Q8N8G9; Q8N9V8; Q8NEH2 

Genbank Protein ID

 AB079366; AK093475; AK096837; AK293616; CR749642; BC031060 

Genbank Nucleotide ID

 BAC81341.1; BAC04179.1; BAC04873.1; BAG57076.1; CAH18436.1; AAH31060.1 

Protein Name

 Piwi-like protein 4 

Protein Synonyms/Alias

  

Gene Name

 PIWIL4 

Gene Synonyms/Alias

 HIWI2; PIWI 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
676	FMTGALNKWYKYNHD	ubiquitination	[1, 2]
679	GALNKWYKYNHDLPA	ubiquitination	[1, 2]

Reference

 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 

Functional Description

 Plays a central role during spermatogenesis by repressing transposable elements and prevent their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons. Directly binds piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements. Associates with secondary piRNAs antisense and PIWIL2/MILI is required for such association. The piRNA process acts upstream of known mediators of DNA methylation. Participates in a piRNA amplification loop. Besides their function in transposable elements repression, piRNAs are probably involved in other processes during meiosis such as translation regulation (By similarity). May be involved in the chromatin-modifying pathway by inducing 'Lys-9' methylation of histone H3 at some loci. 

Sequence Annotation

 DOMAIN 268 384 PAZ.
 DOMAIN 546 838 Piwi.  

Keyword

 Alternative splicing; Complete proteome; Cytoplasm; Developmental protein; Differentiation; Meiosis; Methylation; Nucleus; Polymorphism; Reference proteome; RNA-binding; RNA-mediated gene silencing; Spermatogenesis; Translation regulation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 852 AA 

Protein Sequence

Gene Ontology

 GO:0005634; C:nucleus; ISS:UniProtKB.
 GO:0071547; C:piP-body; ISS:UniProtKB.
 GO:0034584; F:piRNA binding; ISS:UniProtKB.
 GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
 GO:0043046; P:DNA methylation involved in gamete generation; ISS:UniProtKB.
 GO:0031047; P:gene silencing by RNA; ISS:UniProtKB.
 GO:0007126; P:meiosis; IEA:UniProtKB-KW.
 GO:0007275; P:multicellular organismal development; IEA:UniProtKB-KW.
 GO:0034587; P:piRNA metabolic process; ISS:UniProtKB.
 GO:0006417; P:regulation of translation; IEA:UniProtKB-KW.
 GO:0007283; P:spermatogenesis; ISS:UniProtKB. 

Interpro

 IPR003100; PAZ.
 IPR003165; Piwi.
 IPR012337; RNaseH-like_dom. 

Pfam

 PF02170; PAZ
 PF02171; Piwi 

SMART

 SM00949; PAZ
 SM00950; Piwi 

PROSITE

 PS50821; PAZ
 PS50822; PIWI 

PRINTS