CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-013312
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 UV excision repair protein RAD23 homolog B 
Protein Synonyms/Alias
  
Gene Name
 Rad23b 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Rattus norvegicus (Rat) 
NCBI Taxa ID
 10116 
Lysine Modification
Position
Peptide
Type
References
14TLQQQTFKIDIDPEEacetylation[1]
24IDPEETVKALKEKIEacetylation[1]
24IDPEETVKALKEKIEubiquitination[2]
36KIESEKGKDAFPVAGubiquitination[2]
45AFPVAGQKLIYAGKIacetylation[1]
45AFPVAGQKLIYAGKIubiquitination[2]
51QKLIYAGKILSDDTAacetylation[1]
51QKLIYAGKILSDDTAubiquitination[2]
60LSDDTALKEYKIDEKacetylation[1]
60LSDDTALKEYKIDEKubiquitination[2]
63DTALKEYKIDEKNFVacetylation[1]
67KEYKIDEKNFVVVMVubiquitination[2]
76FVVVMVTKPKAVTSAacetylation[1]
76FVVVMVTKPKAVTSAubiquitination[2]
Reference
 [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405]
 [2] Synaptic protein ubiquitination in rat brain revealed by antibody-based ubiquitome analysis.
 Na CH, Jones DR, Yang Y, Wang X, Xu Y, Peng J.
 J Proteome Res. 2012 Sep 7;11(9):4722-32. [PMID: 22871113] 
Functional Description
 Multiubiquitin chain receptor involved in modulation of proteasomal degradation. Binds to polyubiquitin chains. Proposed to be capable to bind simultaneously to the 26S proteasome and to polyubiquitinated substrates and to deliver ubiquitinated proteins to the proteasome. May play a role in endoplasmic reticulum- associated degradation (ERAD) of misfolded glycoproteins by association with PNGase and delivering deglycosylated proteins to the proteasome (By similarity). 
Sequence Annotation
 DOMAIN 1 79 Ubiquitin-like.
 DOMAIN 188 228 UBA 1.
 DOMAIN 274 317 STI1.
 DOMAIN 370 410 UBA 2.
 MOD_RES 155 155 Phosphothreonine (By similarity).
 MOD_RES 160 160 Phosphoserine (By similarity).
 MOD_RES 174 174 Phosphoserine.
 MOD_RES 186 186 Phosphothreonine.
 MOD_RES 199 199 Phosphoserine.
 MOD_RES 202 202 Phosphotyrosine.  
Keyword
 Complete proteome; Cytoplasm; Direct protein sequencing; DNA damage; DNA repair; Nucleus; Phosphoprotein; Proteasome; Reference proteome; Repeat; Ubl conjugation pathway. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 415 AA 
Protein Sequence
MQVTLKTLQQ QTFKIDIDPE ETVKALKEKI ESEKGKDAFP VAGQKLIYAG KILSDDTALK 60
EYKIDEKNFV VVMVTKPKAV TSAVPATTQQ SSSPSTTTVS SSPAAAVAQA PAPTPALAPT 120
STPASTTPAS TTASSEPAPT GATQPEKPAE KPAQTPVLTS PAPADSTPGD SSRSNLFEDA 180
TSALVTGQSY ENMVTEIMSM GYEREQVIAA LRASFNNPDR AVEYLLMGIP GDRESQAVVD 240
PPPQAVSTGT PQSPAVAAAA ATTTATTTTT SGGHPLEFLR NQPQFQQMRQ IIQQNPSLLP 300
ALLQQIGREN PQLLQQISQH QEHFIQMLNE PVQEAGGQGG GGGGGGGGGG GGGGIAEAGS 360
GHMNYIQVTP QEKEAIERLK ALGFPEGLVI QAYFACEKNE NLAANFLLQQ NFDED 415 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0000502; C:proteasome complex; IEA:UniProtKB-KW.
 GO:0071942; C:XPC complex; ISS:UniProtKB.
 GO:0003684; F:damaged DNA binding; IEA:InterPro.
 GO:0000715; P:nucleotide-excision repair, DNA damage recognition; IEA:Compara.
 GO:0043161; P:proteasomal ubiquitin-dependent protein catabolic process; IEA:InterPro.
 GO:0032434; P:regulation of proteasomal ubiquitin-dependent protein catabolic process; IEA:Compara.
 GO:0007283; P:spermatogenesis; IEA:Compara. 
Interpro
 IPR004806; Rad23.
 IPR006636; STI1_HS-bd.
 IPR009060; UBA-like.
 IPR000449; UBA/transl_elong_EF1B_N.
 IPR015940; UBA/transl_elong_EF1B_N_euk.
 IPR000626; Ubiquitin.
 IPR019955; Ubiquitin_supergroup.
 IPR015360; XPC-bd. 
Pfam
 PF00627; UBA
 PF00240; ubiquitin
 PF09280; XPC-binding 
SMART
 SM00727; STI1
 SM00165; UBA
 SM00213; UBQ 
PROSITE
 PS50030; UBA
 PS00299; UBIQUITIN_1
 PS50053; UBIQUITIN_2 
PRINTS
 PR01839; RAD23PROTEIN.