CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-024725
UniProt Accession
Genbank Protein ID
  
Genbank Nucleotide ID
  
Protein Name
 RNA demethylase ALKBH5 
Protein Synonyms/Alias
 Alkylated DNA repair protein alkB homolog 5; Alpha-ketoglutarate-dependent dioxygenase alkB homolog 5 
Gene Name
 Alkbh5 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Rattus norvegicus (Rat) 
NCBI Taxa ID
 10116 
Lysine Modification
Position
Peptide
Type
References
236FGCKFQFKPIRVSEPacetylation[1]
322NTRDPALKPKRSHRKacetylation[1]
Reference
 [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405] 
Functional Description
 Dioxygenase that demethylates RNA by oxidative demethylation: specifically demethylates N(6)-methyladenosine (m(6)A) RNA, the most prevalent internal modification of messenger RNA (mRNA) in higher eukaryotes. Requires molecular oxygen, alpha- ketoglutarate and iron. Demethylation of m(6)A mRNA affects mRNA processing and export and is required for spermatogenesis (By similarity). 
Sequence Annotation
 METAL 205 205 Iron; catalytic (By similarity).
 METAL 207 207 Iron; catalytic (By similarity).
 METAL 267 267 Iron; catalytic (By similarity).
 MOD_RES 2 2 N-acetylalanine (By similarity).
 MOD_RES 65 65 Phosphoserine (By similarity).
 MOD_RES 70 70 Phosphoserine (By similarity).
 MOD_RES 72 72 Phosphotyrosine (By similarity).
 MOD_RES 133 133 N6-acetyllysine (By similarity).
 MOD_RES 362 362 Phosphoserine (By similarity).
 MOD_RES 372 372 Phosphoserine (By similarity).
 MOD_RES 375 375 Phosphoserine (By similarity).
 MOD_RES 385 385 Phosphoserine (By similarity).
 MOD_RES 387 387 Phosphothreonine (By similarity).  
Keyword
 Acetylation; Coiled coil; Complete proteome; Differentiation; Dioxygenase; Iron; Metal-binding; Nucleus; Oxidoreductase; Phosphoprotein; Reference proteome; Spermatogenesis. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 395 AA 
Protein Sequence
MAAASGYTDL REKLKSMTSR DNYKAGSREA AAAAAAAVAA AAAAAAAAEP YPVSGTTKRK 60
YQEDSDPERS DYEEHQLQKE EEARKVKSGI RQIRLFSQDE CSKIEARIDE VVSRAEKGLY 120
NEHTVDRAPL RNKYFFGEGY TYGAQLQKRG PGQERLYPPG DVDEIPEWVH QLVIQKLVEH 180
RVIPEGFVNS AVINDYQPGG CIVSHVDPIH IFERPIVSVS FFSDSALCFG CKFQFKPIRV 240
SEPVLSLPVR RGSVTVLSGY AADEITHCIR PQDIKERRAV IILRKTRLDA PRLETKSLSS 300
STLPPSYASD RLSGNTRDPA LKPKRSHRKA DPDAAHRPRI LEMDKEENRR SVLLPTHRRR 360
GSFSSENYWR KSYESSEDCP EAASSPTRKV KMRRH 395 
Gene Ontology
 GO:0016607; C:nuclear speck; ISS:UniProtKB.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0035515; F:oxidative RNA demethylase activity; ISS:UniProtKB.
 GO:0016706; F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors; ISS:UniProtKB.
 GO:0016702; F:oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen; IEA:UniProtKB-KW.
 GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
 GO:0006406; P:mRNA export from nucleus; ISS:UniProtKB.
 GO:0006397; P:mRNA processing; ISS:UniProtKB.
 GO:0035553; P:oxidative single-stranded RNA demethylation; ISS:UniProtKB.
 GO:0001666; P:response to hypoxia; ISS:UniProtKB.
 GO:0007283; P:spermatogenesis; ISS:UniProtKB. 
Interpro
 IPR027450; AlkB-like. 
Pfam
 PF13532; 2OG-FeII_Oxy_2 
SMART
  
PROSITE
  
PRINTS